A simple and efficient in planta transformation method based on the active regeneration capacity of plants has been developed. This method, named RAPID, utilizes Agrobacterium tumefaciens to deliver genetic material directly into plant meristems, enabling the transformation of plants with strong regeneration capacity. The method involves injecting Agrobacterium into plant stems, which then induces the formation of transformed tissues. These tissues can be vegetatively propagated to produce stable transgenic plants without the need for tissue culture. The RAPID method has been successfully applied to plants such as sweet potato, potato, and bayhops, achieving high transformation efficiency and short duration. It does not require sterile conditions or complex procedures, making it a versatile and efficient method for plant genetic transformation. The method is compatible with various genetic modification strategies, including gene editing, and has shown effectiveness in transforming different plant species. The RAPID method overcomes the limitations of traditional transformation techniques, enabling rapid and efficient genetic modification of plants with active regeneration capacity. The study demonstrates that RAPID can be widely applied to various plant species, offering a promising tool for plant genetic research and biotechnology.A simple and efficient in planta transformation method based on the active regeneration capacity of plants has been developed. This method, named RAPID, utilizes Agrobacterium tumefaciens to deliver genetic material directly into plant meristems, enabling the transformation of plants with strong regeneration capacity. The method involves injecting Agrobacterium into plant stems, which then induces the formation of transformed tissues. These tissues can be vegetatively propagated to produce stable transgenic plants without the need for tissue culture. The RAPID method has been successfully applied to plants such as sweet potato, potato, and bayhops, achieving high transformation efficiency and short duration. It does not require sterile conditions or complex procedures, making it a versatile and efficient method for plant genetic transformation. The method is compatible with various genetic modification strategies, including gene editing, and has shown effectiveness in transforming different plant species. The RAPID method overcomes the limitations of traditional transformation techniques, enabling rapid and efficient genetic modification of plants with active regeneration capacity. The study demonstrates that RAPID can be widely applied to various plant species, offering a promising tool for plant genetic research and biotechnology.