This study investigates the presence and characteristics of microplastics (MPs) in nine different indoor environments of Eskişehir Technical University. MPs were identified through physical characterization using optical microscopy, revealing fragments as the most common type, with colors including black, red, blue, and brown. Chemical and elemental analysis using micro-Raman and SEM-EDX identified 25 types of MPs, including PA 66, PTFE, PP, HDPE, and PE. MPs were found to be airborne, with inhalation rates ranging from 13.88 to 18.51 MPs/m³ and daily intake of 180–240 MPs. The size of MPs varied significantly across environments, with an average size range of ≥2.5–336.89 μm. Common elements detected included C, O, F, Na, Cl, Al, Si, and others. The study highlights the need for further research on the sources, pathways, and health impacts of MPs in indoor environments. It also emphasizes the importance of implementing mitigation strategies, promoting awareness, and developing policies to reduce MP contamination in universities and other indoor spaces. The findings underscore the significance of addressing MP pollution as part of global efforts to combat environmental contamination.This study investigates the presence and characteristics of microplastics (MPs) in nine different indoor environments of Eskişehir Technical University. MPs were identified through physical characterization using optical microscopy, revealing fragments as the most common type, with colors including black, red, blue, and brown. Chemical and elemental analysis using micro-Raman and SEM-EDX identified 25 types of MPs, including PA 66, PTFE, PP, HDPE, and PE. MPs were found to be airborne, with inhalation rates ranging from 13.88 to 18.51 MPs/m³ and daily intake of 180–240 MPs. The size of MPs varied significantly across environments, with an average size range of ≥2.5–336.89 μm. Common elements detected included C, O, F, Na, Cl, Al, Si, and others. The study highlights the need for further research on the sources, pathways, and health impacts of MPs in indoor environments. It also emphasizes the importance of implementing mitigation strategies, promoting awareness, and developing policies to reduce MP contamination in universities and other indoor spaces. The findings underscore the significance of addressing MP pollution as part of global efforts to combat environmental contamination.