This study investigates the effects of three different preparation methods (casting, boiling-casting, and boiling-vacuum) on the properties of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose powder (TOBCP) films. The casting method produced the film with the best electrical properties, as evidenced by its high electrical conductivity and brightness when connected to an LED in a direct current circuit. However, this film had lower tensile strength and thermal stability compared to the films prepared by the boiling and boiling-vacuum methods. The boiling-vacuum film exhibited the most compact fiber structure and the highest tensile and thermal properties, but it showed the lowest electrical properties and the dimmest LED light. These results highlight the potential of using different preparation methods to tailor the properties of TOBCP films for specific applications, such as food packaging. The study provides valuable insights into the relationship between film preparation methods and their impact on the mechanical, thermal, and electrical properties of TOBCP films.This study investigates the effects of three different preparation methods (casting, boiling-casting, and boiling-vacuum) on the properties of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose powder (TOBCP) films. The casting method produced the film with the best electrical properties, as evidenced by its high electrical conductivity and brightness when connected to an LED in a direct current circuit. However, this film had lower tensile strength and thermal stability compared to the films prepared by the boiling and boiling-vacuum methods. The boiling-vacuum film exhibited the most compact fiber structure and the highest tensile and thermal properties, but it showed the lowest electrical properties and the dimmest LED light. These results highlight the potential of using different preparation methods to tailor the properties of TOBCP films for specific applications, such as food packaging. The study provides valuable insights into the relationship between film preparation methods and their impact on the mechanical, thermal, and electrical properties of TOBCP films.