This review article provides a comprehensive overview of the use of natural polymers in bone tissue engineering, focusing on the preparation of nano-scaffolds for bone regeneration. The authors highlight the advantages of using natural polymers, such as chitosan, chitin, cellulose, albumin, and silk fibroin, which are biodegradable, biocompatible, non-toxic, and cost-effective. These polymers are used to create scaffolds that mimic the hierarchical structure of bone, promoting cell migration, proliferation, and differentiation. The review discusses the applications of these natural polymers in various bone-related diseases and injuries, including osteoporosis, fractures, and periodontal regeneration. It also explores the integration of growth factors and bioactive materials to enhance the performance of these scaffolds. The article emphasizes the importance of understanding the physical and chemical properties of these polymers and their impact on cell behavior, as well as the potential of nanotechnology in advancing bone tissue engineering.This review article provides a comprehensive overview of the use of natural polymers in bone tissue engineering, focusing on the preparation of nano-scaffolds for bone regeneration. The authors highlight the advantages of using natural polymers, such as chitosan, chitin, cellulose, albumin, and silk fibroin, which are biodegradable, biocompatible, non-toxic, and cost-effective. These polymers are used to create scaffolds that mimic the hierarchical structure of bone, promoting cell migration, proliferation, and differentiation. The review discusses the applications of these natural polymers in various bone-related diseases and injuries, including osteoporosis, fractures, and periodontal regeneration. It also explores the integration of growth factors and bioactive materials to enhance the performance of these scaffolds. The article emphasizes the importance of understanding the physical and chemical properties of these polymers and their impact on cell behavior, as well as the potential of nanotechnology in advancing bone tissue engineering.