This study investigates the knowledge, attitudes, and practices (KAP) of pharmacy students and faculty members towards artificial intelligence (AI) in six Middle Eastern countries: Jordan, Palestine, Lebanon, Egypt, Saudi Arabia, and Libya. The primary objective is to understand the KAP of these professionals regarding AI in pharmacy practice and to identify predictive factors for their understanding and application of AI in healthcare processes. A total of 875 participants, including 702 students and 173 faculty members, completed an online electronic questionnaire. The results show that while most participants have heard of AI, only a small proportion have a good understanding of its concepts. The overall knowledge level is moderate, with students having a significantly higher score than faculty members. Attitudes towards AI are generally positive, but concerns about job security and patient safety persist. Participants have limited experience using AI tools in their practice, and only a minority have received formal education or training in AI. High income, educational background, and previous technology experience are predictors of KAP. There is a positive correlation between knowledge, attitudes, and practices, suggesting that enhancing knowledge can lead to increased acceptance and adoption of AI in pharmacy practice. The study highlights the need for integrating AI education into pharmacy curricula and addressing concerns about job security and patient safety to ensure responsible and ethical AI integration in pharmacy practice.This study investigates the knowledge, attitudes, and practices (KAP) of pharmacy students and faculty members towards artificial intelligence (AI) in six Middle Eastern countries: Jordan, Palestine, Lebanon, Egypt, Saudi Arabia, and Libya. The primary objective is to understand the KAP of these professionals regarding AI in pharmacy practice and to identify predictive factors for their understanding and application of AI in healthcare processes. A total of 875 participants, including 702 students and 173 faculty members, completed an online electronic questionnaire. The results show that while most participants have heard of AI, only a small proportion have a good understanding of its concepts. The overall knowledge level is moderate, with students having a significantly higher score than faculty members. Attitudes towards AI are generally positive, but concerns about job security and patient safety persist. Participants have limited experience using AI tools in their practice, and only a minority have received formal education or training in AI. High income, educational background, and previous technology experience are predictors of KAP. There is a positive correlation between knowledge, attitudes, and practices, suggesting that enhancing knowledge can lead to increased acceptance and adoption of AI in pharmacy practice. The study highlights the need for integrating AI education into pharmacy curricula and addressing concerns about job security and patient safety to ensure responsible and ethical AI integration in pharmacy practice.