Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases

Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases

Received: January 5, 2009 | Yasuyoshi Mori · Tsugunori Notomi
The article reviews the development, current status, and future prospects of Loop-mediated isothermal amplification (LAMP) technology for the diagnosis of infectious diseases. LAMP is a rapid, accurate, and cost-effective nucleic acid amplification method that has been developed into commercially available detection kits for various pathogens, including bacteria and viruses. The technology is particularly useful in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The article highlights the historical development of LAMP, its application in detecting various pathogens such as Salmonella, Escherichia coli, noroviruses, influenza, and malaria, and its potential for use in point-of-care testing. The future prospects of LAMP include the integration with microfluidic technologies and the development of Lab-on-a-chip devices, which could further enhance its diagnostic capabilities and accessibility.The article reviews the development, current status, and future prospects of Loop-mediated isothermal amplification (LAMP) technology for the diagnosis of infectious diseases. LAMP is a rapid, accurate, and cost-effective nucleic acid amplification method that has been developed into commercially available detection kits for various pathogens, including bacteria and viruses. The technology is particularly useful in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The article highlights the historical development of LAMP, its application in detecting various pathogens such as Salmonella, Escherichia coli, noroviruses, influenza, and malaria, and its potential for use in point-of-care testing. The future prospects of LAMP include the integration with microfluidic technologies and the development of Lab-on-a-chip devices, which could further enhance its diagnostic capabilities and accessibility.
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[slides and audio] Loop-mediated isothermal ampli%EF%AC%81 cation (LAMP)%3A a rapid%2C accurate%2C and cost-effective diagnostic method for infectious diseases