This review discusses the molecular aspects of the functioning of pathogenic bacteria biofilms based on quorum sensing (QS) signal-response systems and innovative non-antibiotic strategies for their elimination. Quorum sensing is a bacterial communication system that enables cells to regulate gene expression in response to population density, using small signaling molecules called autoinducers (AIs). QS is crucial for biofilm formation, virulence, and antibiotic resistance. Inhibiting QS, known as quorum quenching (QQ), offers an alternative to traditional antibiotics for treating microbial infections. The review summarizes recent progress in biofilm research, focusing on mechanisms of biofilm resistance to antibiotics and alternative strategies to control AMR and biofilm formation. It discusses the role of QS in global gene expression regulation, the different QS systems in Gram-negative and Gram-positive bacteria, and the various classes of antimicrobial compounds used to control biofilm formation. The review also highlights the potential of QS inhibitors, such as enzymatic QQ, antimicrobial peptides, and other innovative strategies, for effectively eradicating biofilm-related infections. The review emphasizes the importance of understanding QS mechanisms and developing non-antibiotic strategies to combat biofilm-associated infections.This review discusses the molecular aspects of the functioning of pathogenic bacteria biofilms based on quorum sensing (QS) signal-response systems and innovative non-antibiotic strategies for their elimination. Quorum sensing is a bacterial communication system that enables cells to regulate gene expression in response to population density, using small signaling molecules called autoinducers (AIs). QS is crucial for biofilm formation, virulence, and antibiotic resistance. Inhibiting QS, known as quorum quenching (QQ), offers an alternative to traditional antibiotics for treating microbial infections. The review summarizes recent progress in biofilm research, focusing on mechanisms of biofilm resistance to antibiotics and alternative strategies to control AMR and biofilm formation. It discusses the role of QS in global gene expression regulation, the different QS systems in Gram-negative and Gram-positive bacteria, and the various classes of antimicrobial compounds used to control biofilm formation. The review also highlights the potential of QS inhibitors, such as enzymatic QQ, antimicrobial peptides, and other innovative strategies, for effectively eradicating biofilm-related infections. The review emphasizes the importance of understanding QS mechanisms and developing non-antibiotic strategies to combat biofilm-associated infections.