Mouse Genome Informatics (MGI) is a comprehensive, expertly curated knowledgebase that supports research on the laboratory mouse, focusing on genetic and genomic aspects of human biology and disease. MGI comprises several integrated resources, with the Mouse Genome Database (MGD) and Gene Expression Database (GXD) as core components. MGI serves as a central community resource, providing integrated information about mouse genome features, variation, expression, gene function, phenotype, and human disease models. The resource is accessible through various search tools, including the Quick Search tool, which has been modernized to improve usability and performance. MGI uses controlled metadata vocabularies and biological ontologies to standardize data, ensuring comprehensive and FAIR-compliant data access. Recent enhancements include improvements to the literature acquisition process, a renovated Quick Search tool, expanded regulatory feature representation, new cell type annotations for expression data, and updates to mutation/gene relationships and the Human-Mouse: Disease Connection (HMDC) resource. MGI also maintains a catalog of mouse alleles, mutations, and strains, with over 100,547 alleles and 691,056 mutant alleles cataloged. The resource is widely used by basic scientists, translational researchers, and data scientists, contributing to advancements in understanding human health and disease using the mouse as a model system.Mouse Genome Informatics (MGI) is a comprehensive, expertly curated knowledgebase that supports research on the laboratory mouse, focusing on genetic and genomic aspects of human biology and disease. MGI comprises several integrated resources, with the Mouse Genome Database (MGD) and Gene Expression Database (GXD) as core components. MGI serves as a central community resource, providing integrated information about mouse genome features, variation, expression, gene function, phenotype, and human disease models. The resource is accessible through various search tools, including the Quick Search tool, which has been modernized to improve usability and performance. MGI uses controlled metadata vocabularies and biological ontologies to standardize data, ensuring comprehensive and FAIR-compliant data access. Recent enhancements include improvements to the literature acquisition process, a renovated Quick Search tool, expanded regulatory feature representation, new cell type annotations for expression data, and updates to mutation/gene relationships and the Human-Mouse: Disease Connection (HMDC) resource. MGI also maintains a catalog of mouse alleles, mutations, and strains, with over 100,547 alleles and 691,056 mutant alleles cataloged. The resource is widely used by basic scientists, translational researchers, and data scientists, contributing to advancements in understanding human health and disease using the mouse as a model system.