This study presents an integrated 3D bioprinted scaffold designed to facilitate real-time monitoring of bone regeneration and implant degradation. The scaffold, denoted as C@M/GLU, incorporates laponite (Lap), CyP-loaded mesoporous silica (CyP/MSNs), and ultrasmall superparamagnetic iron oxide nanoparticles (USPIO/SSIO) into a bioink containing bone marrow mesenchymal stem cells (BMSCs). The composite scaffold enhances bone regeneration through the controlled release of silicon (Si) and magnesium (Mg) ions. Near-infrared fluorescence (NIR-FL) imaging is used to monitor alkaline phosphate (ALP) expression, providing an accurate reflection of osteogenic activity. Magnetic resonance (MR) imaging is employed to track the degradation of the scaffold by monitoring changes in magnetic resonance signals. Both in vitro and in vivo experiments demonstrate the effectiveness of the scaffold in enhancing bone regeneration and accurately monitoring the bone repair process. The study highlights the potential of this multi-modal imaging scaffold for in situ bone implant applications.This study presents an integrated 3D bioprinted scaffold designed to facilitate real-time monitoring of bone regeneration and implant degradation. The scaffold, denoted as C@M/GLU, incorporates laponite (Lap), CyP-loaded mesoporous silica (CyP/MSNs), and ultrasmall superparamagnetic iron oxide nanoparticles (USPIO/SSIO) into a bioink containing bone marrow mesenchymal stem cells (BMSCs). The composite scaffold enhances bone regeneration through the controlled release of silicon (Si) and magnesium (Mg) ions. Near-infrared fluorescence (NIR-FL) imaging is used to monitor alkaline phosphate (ALP) expression, providing an accurate reflection of osteogenic activity. Magnetic resonance (MR) imaging is employed to track the degradation of the scaffold by monitoring changes in magnetic resonance signals. Both in vitro and in vivo experiments demonstrate the effectiveness of the scaffold in enhancing bone regeneration and accurately monitoring the bone repair process. The study highlights the potential of this multi-modal imaging scaffold for in situ bone implant applications.