Superporous sponge prepared by secondary network compaction with enhanced permeability and mechanical properties for non-compressible hemostasis in pigs

Superporous sponge prepared by secondary network compaction with enhanced permeability and mechanical properties for non-compressible hemostasis in pigs

27 June 2024 | Tianshen Jiang, Sirong Chen, Jingwen Xu, Yuxiao Zhang, Hao Fu, Qiangjun Ling, Yan Xu, Xiangyu Chu, Ruinan Wang, Liangcong Hu, Hao Li, Weitong Huang, Liming Bian, Pengchao Zhao, Fuxin Wei
This study presents a novel method for fabricating superporous chitosan sponges with enhanced permeability and mechanical properties, designed to achieve rapid hemostasis in non-compressible hemorrhage. The sponges are prepared using a temperature-assisted secondary network compaction (TA-2ndNC) strategy, which involves phase separation-induced primary compaction followed by secondary compaction at an optimal temperature of 0°C. This approach results in highly interconnected porous structures with large pore sizes, high porosity, and enhanced network density. The sponges exhibit rapid shape recovery after absorbing blood, maintaining sufficient pressure on wounds to form a robust physical barrier, thereby improving hemostatic efficiency. Compared to commercial gauze, gelatin sponges, and chitosan powder, the alkylated superporous chitosan sponges (A-spCS) demonstrate superior hemostatic capabilities, promoting cell infiltration, vascular regeneration, and in-situ tissue regeneration in non-compressible organ injury models. The A-spCS also shows good biocompatibility and antibacterial properties, making it a promising candidate for clinical applications in hemostasis settings.This study presents a novel method for fabricating superporous chitosan sponges with enhanced permeability and mechanical properties, designed to achieve rapid hemostasis in non-compressible hemorrhage. The sponges are prepared using a temperature-assisted secondary network compaction (TA-2ndNC) strategy, which involves phase separation-induced primary compaction followed by secondary compaction at an optimal temperature of 0°C. This approach results in highly interconnected porous structures with large pore sizes, high porosity, and enhanced network density. The sponges exhibit rapid shape recovery after absorbing blood, maintaining sufficient pressure on wounds to form a robust physical barrier, thereby improving hemostatic efficiency. Compared to commercial gauze, gelatin sponges, and chitosan powder, the alkylated superporous chitosan sponges (A-spCS) demonstrate superior hemostatic capabilities, promoting cell infiltration, vascular regeneration, and in-situ tissue regeneration in non-compressible organ injury models. The A-spCS also shows good biocompatibility and antibacterial properties, making it a promising candidate for clinical applications in hemostasis settings.
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