The supplementary materials for the article "1.63-billion-year-old multicellular eukaryotes from the Chuanlinggou Formation in North China" by Lanyun Miao et al. include a variety of figures, tables, and data supporting the main findings. These materials provide detailed geological context, morphological and ultrastructural analyses, and chemical analyses of the fossilized eukaryotic algae *Qingshania magnifica*. The figures illustrate the stratigraphy and geological map of the study area, transmitted-light photomicrographs and SEM images of *Qingshania magnifica* and other co-occurring taxa, and morphometric analyses. The tables contain measurements of selected eukaryotic algae and filamentous bacteria, FTIR band assignments, and distribution of multicellularity in the Eukarya. The data sections provide measurements and size frequency of *Qingshania magnifica*, Raman and FTIR data, and measurements of other fossil taxa. The references cited cover a wide range of topics related to eukaryotic evolution, fossilization, and microbial ecology.The supplementary materials for the article "1.63-billion-year-old multicellular eukaryotes from the Chuanlinggou Formation in North China" by Lanyun Miao et al. include a variety of figures, tables, and data supporting the main findings. These materials provide detailed geological context, morphological and ultrastructural analyses, and chemical analyses of the fossilized eukaryotic algae *Qingshania magnifica*. The figures illustrate the stratigraphy and geological map of the study area, transmitted-light photomicrographs and SEM images of *Qingshania magnifica* and other co-occurring taxa, and morphometric analyses. The tables contain measurements of selected eukaryotic algae and filamentous bacteria, FTIR band assignments, and distribution of multicellularity in the Eukarya. The data sections provide measurements and size frequency of *Qingshania magnifica*, Raman and FTIR data, and measurements of other fossil taxa. The references cited cover a wide range of topics related to eukaryotic evolution, fossilization, and microbial ecology.