SMART (Simple Modular Architecture Research Tool) is a web resource for identifying and annotating protein domains and analyzing protein domain architectures. Version 8 of SMART includes manually curated models for over 1300 protein domains, with approximately 100 new models added since the last update. The underlying protein databases have been synchronized with UniProt, Ensembl, and STRING, doubling the total number of annotated domains to over 200 million. Key improvements include: 1. **Expanded Domain Coverage**: Over 100 new domains have been added, bringing the total to 1302. 2. **Updated Protein Databases**: The main database combines UniProt and all stable Ensembl proteomes, covering >50 million proteins from ~460 thousand species. 3. **New Protein Visualization Engine**: A vector-based display engine for protein schematics, using SVG images that scale seamlessly and can be zoomed without loss in quality. 4. **Updated Annotation Pages**: Streamlined and updated information sources, including protein orthology data from eggNOG, posttranslational modifications from PTMcode, and protein interaction data from STRING. 5. **Optimized Search Engine**: An updated full-text search engine with increased search speed and access to more text information. 6. **Taxonomic Tree Data Export**: Improved export options for phylogenetic trees, compatible with the Interactive Tree of Life (iTOL). These updates enhance the user experience and maintain SMART's status as a widely used tool for protein domain analysis.SMART (Simple Modular Architecture Research Tool) is a web resource for identifying and annotating protein domains and analyzing protein domain architectures. Version 8 of SMART includes manually curated models for over 1300 protein domains, with approximately 100 new models added since the last update. The underlying protein databases have been synchronized with UniProt, Ensembl, and STRING, doubling the total number of annotated domains to over 200 million. Key improvements include: 1. **Expanded Domain Coverage**: Over 100 new domains have been added, bringing the total to 1302. 2. **Updated Protein Databases**: The main database combines UniProt and all stable Ensembl proteomes, covering >50 million proteins from ~460 thousand species. 3. **New Protein Visualization Engine**: A vector-based display engine for protein schematics, using SVG images that scale seamlessly and can be zoomed without loss in quality. 4. **Updated Annotation Pages**: Streamlined and updated information sources, including protein orthology data from eggNOG, posttranslational modifications from PTMcode, and protein interaction data from STRING. 5. **Optimized Search Engine**: An updated full-text search engine with increased search speed and access to more text information. 6. **Taxonomic Tree Data Export**: Improved export options for phylogenetic trees, compatible with the Interactive Tree of Life (iTOL). These updates enhance the user experience and maintain SMART's status as a widely used tool for protein domain analysis.