This review discusses the application of two-dimensional (2D) materials in advanced electronic biosensors for point-of-care devices. The article highlights the potential of biosensors based on field-effect transistors (FETs) using materials such as graphene, transition metal dichalcogenides (TMDCs), and black phosphorus. These biosensors offer advantages like low detection limits, real-time monitoring, label-free diagnosis, and high selectivity. The review covers the operational mechanisms and detection capabilities of biosensing devices utilizing various 2D materials, as well as their diverse applications in conventional and wearable devices. It also addresses the limitations and challenges of these devices, along with future prospects and advancements. A detailed comparison of FET-based biosensors is provided, along with various other biosensing platforms and their working mechanisms. The review aims to stimulate further research and innovation in this field while educating the scientific community about the latest advancements in 2D materials-based biosensors. The article also discusses the basic assembly of FET biosensors, their working mechanisms, and the use of 2D materials in biosensing applications. It highlights the potential of 2D materials in biosensing technology, emphasizing their unique properties and advantages. The review also explores the use of specific 2D materials such as graphene and TMDCs in biosensors for detecting various biomolecules, including proteins and pathogens. The study discusses the fabrication and characterization of biosensors using these materials, as well as their performance in detecting target analytes. The review concludes with a comprehensive assessment of the potential and challenges of 2D materials-based biosensors, emphasizing their significance in advancing biosensing technology.This review discusses the application of two-dimensional (2D) materials in advanced electronic biosensors for point-of-care devices. The article highlights the potential of biosensors based on field-effect transistors (FETs) using materials such as graphene, transition metal dichalcogenides (TMDCs), and black phosphorus. These biosensors offer advantages like low detection limits, real-time monitoring, label-free diagnosis, and high selectivity. The review covers the operational mechanisms and detection capabilities of biosensing devices utilizing various 2D materials, as well as their diverse applications in conventional and wearable devices. It also addresses the limitations and challenges of these devices, along with future prospects and advancements. A detailed comparison of FET-based biosensors is provided, along with various other biosensing platforms and their working mechanisms. The review aims to stimulate further research and innovation in this field while educating the scientific community about the latest advancements in 2D materials-based biosensors. The article also discusses the basic assembly of FET biosensors, their working mechanisms, and the use of 2D materials in biosensing applications. It highlights the potential of 2D materials in biosensing technology, emphasizing their unique properties and advantages. The review also explores the use of specific 2D materials such as graphene and TMDCs in biosensors for detecting various biomolecules, including proteins and pathogens. The study discusses the fabrication and characterization of biosensors using these materials, as well as their performance in detecting target analytes. The review concludes with a comprehensive assessment of the potential and challenges of 2D materials-based biosensors, emphasizing their significance in advancing biosensing technology.