This review article explores the potential of two-dimensional (2D) materials in advanced electronic biosensors for point-of-care (PoC) devices. It highlights the operational mechanisms and detection capabilities of biosensing devices utilizing graphene, transition metal dichalcogenides (TMDCs), black phosphorus, and other 2D materials. The incorporation of these materials into field-effect transistor (FET)-based biosensors offers significant advantages, including low detection limits, real-time monitoring, label-free diagnosis, and exceptional selectivity. The review also discusses the diverse applications of these biosensors, ranging from conventional to wearable devices, and assesses the limitations and challenges faced by these devices. Additionally, it provides a detailed comparison of FET-based biosensors with other biosensing platforms and their working mechanisms. The ultimate goal is to stimulate further research and innovation in this field while educating the scientific community about the latest advancements in 2D materials-based biosensors.This review article explores the potential of two-dimensional (2D) materials in advanced electronic biosensors for point-of-care (PoC) devices. It highlights the operational mechanisms and detection capabilities of biosensing devices utilizing graphene, transition metal dichalcogenides (TMDCs), black phosphorus, and other 2D materials. The incorporation of these materials into field-effect transistor (FET)-based biosensors offers significant advantages, including low detection limits, real-time monitoring, label-free diagnosis, and exceptional selectivity. The review also discusses the diverse applications of these biosensors, ranging from conventional to wearable devices, and assesses the limitations and challenges faced by these devices. Additionally, it provides a detailed comparison of FET-based biosensors with other biosensing platforms and their working mechanisms. The ultimate goal is to stimulate further research and innovation in this field while educating the scientific community about the latest advancements in 2D materials-based biosensors.