This review article discusses the principles, materials, and applications of 3D bioprinting, a technology used to create complex 3D functional living tissues or artificial organs by precisely dispensing cell-laden biomaterials. Despite its early stages, bioprinting has shown potential in regenerative medicine for generating transplantable tissues such as skin, cartilage, and bone. However, it faces technical challenges in high-resolution cell deposition, controlled cell distribution, vascularization, and innervation within complex 3D tissues. The article highlights the advantages of bioprinting, including accurate cell distribution, high-resolution deposition, scalability, and cost-effectiveness. It also reviews the three major bioprinting techniques: inkjet, laser-assisted, and extrusion, each with specific strengths and limitations. The review further discusses the materials used in bioprinting, such as hydrogels and cells, and their properties. Additionally, it explores the current applications of bioprinting, including the creation of vascular features, bone and cartilage tissue engineering, neuronal tissues, and drug screening systems. Finally, the article addresses the present limitations of bioprinting, such as the difficulty in printing complex hollow structures and the lack of reliable methods for pre-vascularized tissue printing, and outlines future prospects, emphasizing the potential of bioprinting in nano-biofabrication and artificial organ generation.This review article discusses the principles, materials, and applications of 3D bioprinting, a technology used to create complex 3D functional living tissues or artificial organs by precisely dispensing cell-laden biomaterials. Despite its early stages, bioprinting has shown potential in regenerative medicine for generating transplantable tissues such as skin, cartilage, and bone. However, it faces technical challenges in high-resolution cell deposition, controlled cell distribution, vascularization, and innervation within complex 3D tissues. The article highlights the advantages of bioprinting, including accurate cell distribution, high-resolution deposition, scalability, and cost-effectiveness. It also reviews the three major bioprinting techniques: inkjet, laser-assisted, and extrusion, each with specific strengths and limitations. The review further discusses the materials used in bioprinting, such as hydrogels and cells, and their properties. Additionally, it explores the current applications of bioprinting, including the creation of vascular features, bone and cartilage tissue engineering, neuronal tissues, and drug screening systems. Finally, the article addresses the present limitations of bioprinting, such as the difficulty in printing complex hollow structures and the lack of reliable methods for pre-vascularized tissue printing, and outlines future prospects, emphasizing the potential of bioprinting in nano-biofabrication and artificial organ generation.