8-hydroxy-2'-deoxyguanosine (8-OHdG) is a major biomarker of oxidative DNA damage and is widely used to assess oxidative stress and carcinogenesis. It is formed when hydroxyl radicals attack DNA, leading to the formation of 8-oxodG, which is a common lesion in DNA. 8-OHdG is detected in urine and is used as a biomarker for various cancers and degenerative diseases. It is measured using methods such as high-performance liquid chromatography (HPLC) with electrochemical detection, gas chromatography-mass spectrometry (GC-MS), and HPLC tandem mass spectrometry. The European Standards Committee for Oxidative DNA Damage was established to address methodological issues in measuring 8-OHdG. 8-OHdG is also used to estimate DNA damage in humans after exposure to carcinogens such as tobacco smoke, asbestos, and polycyclic aromatic hydrocarbons. Recent studies have shown that 8-OHdG is a significant biomarker for oxidative DNA damage and is associated with various diseases, including cancer. The levels of 8-OHdG are influenced by factors such as lifestyle, diet, and exposure to environmental pollutants. 8-OHdG is also used in epidemiological studies to assess cancer risk and oxidative DNA damage. The levels of 8-OHdG in DNA are affected by endogenous and exogenous factors, and its measurement is important for understanding the mechanisms of carcinogenesis and oxidative stress. The use of 8-OHdG as a biomarker has increased in recent years, and it is considered an important tool for assessing oxidative DNA damage and its role in cancer development.8-hydroxy-2'-deoxyguanosine (8-OHdG) is a major biomarker of oxidative DNA damage and is widely used to assess oxidative stress and carcinogenesis. It is formed when hydroxyl radicals attack DNA, leading to the formation of 8-oxodG, which is a common lesion in DNA. 8-OHdG is detected in urine and is used as a biomarker for various cancers and degenerative diseases. It is measured using methods such as high-performance liquid chromatography (HPLC) with electrochemical detection, gas chromatography-mass spectrometry (GC-MS), and HPLC tandem mass spectrometry. The European Standards Committee for Oxidative DNA Damage was established to address methodological issues in measuring 8-OHdG. 8-OHdG is also used to estimate DNA damage in humans after exposure to carcinogens such as tobacco smoke, asbestos, and polycyclic aromatic hydrocarbons. Recent studies have shown that 8-OHdG is a significant biomarker for oxidative DNA damage and is associated with various diseases, including cancer. The levels of 8-OHdG are influenced by factors such as lifestyle, diet, and exposure to environmental pollutants. 8-OHdG is also used in epidemiological studies to assess cancer risk and oxidative DNA damage. The levels of 8-OHdG in DNA are affected by endogenous and exogenous factors, and its measurement is important for understanding the mechanisms of carcinogenesis and oxidative stress. The use of 8-OHdG as a biomarker has increased in recent years, and it is considered an important tool for assessing oxidative DNA damage and its role in cancer development.