The absorption of light by chlorophyll solutions is influenced by the solvent used. The study compares absorption coefficients of chlorophylls a and b in different solvents, including anhydrous ether, methanol, and aqueous acetone. The results show that the solvent significantly affects the absorption coefficients, with variations in the values observed depending on the solvent. For example, in methanol, the absorption maximum for chlorophyll a is greater than the blue peak, while in ether, the values for chlorophyll a at 6600 Å are about 4% higher than previously reported. The study also notes that the solvent's effect on the absorption maxima for both chlorophylls a and b can be significant, especially when moving from anhydrous acetone to aqueous acetone. The study emphasizes the importance of using solvents that closely match those used in the extracts to ensure accurate comparisons. It also discusses the potential for errors due to the use of highly purified solvents, especially when the solutions are used as standards for comparison with extracts. The study concludes that solid chlorophylls a and b, when dissolved in a suitable solvent, can be used to account for the entire absorption curve in the visible part of the spectrum, provided that the solvent is chosen appropriately. The study also examines the determination of chlorophylls a and b in plant extracts, using simultaneous equations based on the absorption coefficients at specific wavelengths. The results show that the absorption of chlorophyll a is dominant at 6630 Å, while chlorophyll b has a greater effect at 6450 Å. The study finds that the ratios of chlorophylls a to b in Avena and Malva are 2.84 and 3.18, respectively. The study also notes that the divergence from 5400 to 4250 Å is solely due to carotenoids. The study concludes that chlorophylls as described by Willstätter and Stoll are artifacts only in the sense that they have been liberated from the pigment-protein complex termed chloroplastin.The absorption of light by chlorophyll solutions is influenced by the solvent used. The study compares absorption coefficients of chlorophylls a and b in different solvents, including anhydrous ether, methanol, and aqueous acetone. The results show that the solvent significantly affects the absorption coefficients, with variations in the values observed depending on the solvent. For example, in methanol, the absorption maximum for chlorophyll a is greater than the blue peak, while in ether, the values for chlorophyll a at 6600 Å are about 4% higher than previously reported. The study also notes that the solvent's effect on the absorption maxima for both chlorophylls a and b can be significant, especially when moving from anhydrous acetone to aqueous acetone. The study emphasizes the importance of using solvents that closely match those used in the extracts to ensure accurate comparisons. It also discusses the potential for errors due to the use of highly purified solvents, especially when the solutions are used as standards for comparison with extracts. The study concludes that solid chlorophylls a and b, when dissolved in a suitable solvent, can be used to account for the entire absorption curve in the visible part of the spectrum, provided that the solvent is chosen appropriately. The study also examines the determination of chlorophylls a and b in plant extracts, using simultaneous equations based on the absorption coefficients at specific wavelengths. The results show that the absorption of chlorophyll a is dominant at 6630 Å, while chlorophyll b has a greater effect at 6450 Å. The study finds that the ratios of chlorophylls a to b in Avena and Malva are 2.84 and 3.18, respectively. The study also notes that the divergence from 5400 to 4250 Å is solely due to carotenoids. The study concludes that chlorophylls as described by Willstätter and Stoll are artifacts only in the sense that they have been liberated from the pigment-protein complex termed chloroplastin.