The article introduces the Antibiotic Resistance Genes Database (ARDB), a manually curated database designed to facilitate the identification and characterization of antibiotic resistance genes. The database unifies publicly available information on antibiotic resistance, providing rich annotations for each gene and resistance type, including resistance profiles, mechanisms of action, ontology, COG and CDD annotations, and external links to sequence and protein databases. ARDB supports sequence similarity searches and includes a tool for characterizing common mutations that confer antibiotic resistance. Currently, ARDB contains data on 13,293 genes, 377 types, 257 antibiotics, 632 genomes, 933 species, and 124 genera. The database aims to address the limitations of existing resources and provide a comprehensive compendium of antibiotic resistance factors, useful for researchers in microbiology, clinical medicine, and bio-defense. The article also discusses the construction of ARDB, its content, and the development of an ontology for annotating antibiotic resistance information.The article introduces the Antibiotic Resistance Genes Database (ARDB), a manually curated database designed to facilitate the identification and characterization of antibiotic resistance genes. The database unifies publicly available information on antibiotic resistance, providing rich annotations for each gene and resistance type, including resistance profiles, mechanisms of action, ontology, COG and CDD annotations, and external links to sequence and protein databases. ARDB supports sequence similarity searches and includes a tool for characterizing common mutations that confer antibiotic resistance. Currently, ARDB contains data on 13,293 genes, 377 types, 257 antibiotics, 632 genomes, 933 species, and 124 genera. The database aims to address the limitations of existing resources and provide a comprehensive compendium of antibiotic resistance factors, useful for researchers in microbiology, clinical medicine, and bio-defense. The article also discusses the construction of ARDB, its content, and the development of an ontology for annotating antibiotic resistance information.