VOLUME 19, 1963 | M. C. LEDBETTER, Ph.D., and K. R. PORTER, Ph.D.
This paper reports the electron microscopic examination of the cortices of plant cells involved in wall formation, using glutaraldehyde as a fixative. The authors found that this method significantly improved the preservation of cortical fine structures compared to previous studies using OsO4 alone. They observed slender tubules, 230 to 270 Å in diameter and of indeterminate length, in the cortical regions of cells from two angiosperms and one gymnosperm. These tubules are morphologically similar to those found in mitotic spindles but are slightly smaller. The tubules are positioned near the plasma membrane and are oriented circumferentially around the lateral walls of the cell, mirroring the orientation of cellulose microfibrils in the adjacent cell walls. The authors suggest that these tubules may play a role in governing cytoplasmic streaming and influencing the disposition of cell wall materials. The study also discusses the potential significance of these tubules in the development and orientation of cellulose microfibrils.This paper reports the electron microscopic examination of the cortices of plant cells involved in wall formation, using glutaraldehyde as a fixative. The authors found that this method significantly improved the preservation of cortical fine structures compared to previous studies using OsO4 alone. They observed slender tubules, 230 to 270 Å in diameter and of indeterminate length, in the cortical regions of cells from two angiosperms and one gymnosperm. These tubules are morphologically similar to those found in mitotic spindles but are slightly smaller. The tubules are positioned near the plasma membrane and are oriented circumferentially around the lateral walls of the cell, mirroring the orientation of cellulose microfibrils in the adjacent cell walls. The authors suggest that these tubules may play a role in governing cytoplasmic streaming and influencing the disposition of cell wall materials. The study also discusses the potential significance of these tubules in the development and orientation of cellulose microfibrils.