A STUDY OF FIXATION FOR ELECTRON MICROSCOPY

A STUDY OF FIXATION FOR ELECTRON MICROSCOPY

(Received for publication, November 5, 1951) | G. E. PALADE, M.D.
This study by G. E. Palade, published in 1951, focuses on the improvement of fixation techniques for electron microscopy. The author highlights the importance of proper fixation to achieve accurate morphological information, particularly in fine structures. Traditional methods, such as using OsO4 vapor, have been found to influence cell morphology, often leading to poor results. Palade's experiments suggest that the quality of OsO4 fixation is highly dependent on the pH of the solution, with optimal results achieved at pH 7.3-7.5 using acetate-veronal buffer. The study also indicates that tissue acidification precedes OsO4 fixation, and this acidification can be prevented by buffering the fixative. Palade recommends using 1% OsO4 buffered at pH 7.3-7.5 with acetate-veronal buffer as an appropriate fixative for electron microscopy. The findings provide a significant advancement in the field, improving the reliability and quality of electron microscopic studies.This study by G. E. Palade, published in 1951, focuses on the improvement of fixation techniques for electron microscopy. The author highlights the importance of proper fixation to achieve accurate morphological information, particularly in fine structures. Traditional methods, such as using OsO4 vapor, have been found to influence cell morphology, often leading to poor results. Palade's experiments suggest that the quality of OsO4 fixation is highly dependent on the pH of the solution, with optimal results achieved at pH 7.3-7.5 using acetate-veronal buffer. The study also indicates that tissue acidification precedes OsO4 fixation, and this acidification can be prevented by buffering the fixative. Palade recommends using 1% OsO4 buffered at pH 7.3-7.5 with acetate-veronal buffer as an appropriate fixative for electron microscopy. The findings provide a significant advancement in the field, improving the reliability and quality of electron microscopic studies.
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