A technique for ultracryotomy of cell suspensions and tissues is described. The method involves infusing fixed tissue pieces with sucrose before freezing to control sectioning consistency. Sucrose allows for the ultracryotomy of various tissues and single cells in suspension, preserving ultrastructural detail comparable to conventional embedding. The technique uses a small droplet of a saturated or near-saturated sucrose solution to transfer frozen sections onto a grid or water surface. Upon melting, sections spread flat and smooth due to surface tension, allowing individual cell sections to remain confined. This method avoids flotation on dimethyl sulfoxide solutions and enables the cutting of wide dry sections. Negative staining with 0.2–0.5% neutral phosphotungstic acid (PTA) or 0.5% uranyl acetate (UA) is found to be more suitable for ultrastructural preservation in frozen sections than positive staining. The technique is illustrated with various tissue preparations and suspensions of erythrocytes and bacterial cells. The method is effective for preserving structural details in frozen sections, with results comparable to or better than conventional sections. The technique is particularly useful for cytochemical and immunocytochemical procedures due to its ability to retain antigenic and enzymatic activities of macromolecules. The study highlights the advantages of ultracryotomy over conventional ultramicrotomy, including the preservation of structural details and the ability to avoid artifacts from embedding processes. The technique is supported by references to previous studies and is recommended for ultrastructural studies.A technique for ultracryotomy of cell suspensions and tissues is described. The method involves infusing fixed tissue pieces with sucrose before freezing to control sectioning consistency. Sucrose allows for the ultracryotomy of various tissues and single cells in suspension, preserving ultrastructural detail comparable to conventional embedding. The technique uses a small droplet of a saturated or near-saturated sucrose solution to transfer frozen sections onto a grid or water surface. Upon melting, sections spread flat and smooth due to surface tension, allowing individual cell sections to remain confined. This method avoids flotation on dimethyl sulfoxide solutions and enables the cutting of wide dry sections. Negative staining with 0.2–0.5% neutral phosphotungstic acid (PTA) or 0.5% uranyl acetate (UA) is found to be more suitable for ultrastructural preservation in frozen sections than positive staining. The technique is illustrated with various tissue preparations and suspensions of erythrocytes and bacterial cells. The method is effective for preserving structural details in frozen sections, with results comparable to or better than conventional sections. The technique is particularly useful for cytochemical and immunocytochemical procedures due to its ability to retain antigenic and enzymatic activities of macromolecules. The study highlights the advantages of ultracryotomy over conventional ultramicrotomy, including the preservation of structural details and the ability to avoid artifacts from embedding processes. The technique is supported by references to previous studies and is recommended for ultrastructural studies.