This paper presents a technique for ultracryotomy of fixed tissue and single cells in suspension, which allows for the preservation and visualization of ultrastructural detail equivalent to conventional embedding procedures. The method involves infusing sucrose into glutaraldehyde-fixed tissue pieces before freezing to control the sectioning consistency. By adjusting the sucrose concentration and sectioning temperature, a wide range of tissues can be smoothly sectioned. Isolated cells suspended in sucrose solution are sectioned by cutting the frozen droplet. The sections are transferred from the knife edge to a grid substrate or water surface using a small liquid droplet of a saturated or near-saturated sucrose solution. This technique overcomes the limitations of previous methods, such as the high viscosity of flotation liquids and the chemical effects of dimethyl sulfoxide (DMSO). The use of negative staining is found to be more suitable for ultrastructural preservation in frozen sections compared to positive staining. The technique is demonstrated with various tissue preparations and suspensions of erythrocytes and bacterial cells, showing comparable or better morphological detail than conventional sections.This paper presents a technique for ultracryotomy of fixed tissue and single cells in suspension, which allows for the preservation and visualization of ultrastructural detail equivalent to conventional embedding procedures. The method involves infusing sucrose into glutaraldehyde-fixed tissue pieces before freezing to control the sectioning consistency. By adjusting the sucrose concentration and sectioning temperature, a wide range of tissues can be smoothly sectioned. Isolated cells suspended in sucrose solution are sectioned by cutting the frozen droplet. The sections are transferred from the knife edge to a grid substrate or water surface using a small liquid droplet of a saturated or near-saturated sucrose solution. This technique overcomes the limitations of previous methods, such as the high viscosity of flotation liquids and the chemical effects of dimethyl sulfoxide (DMSO). The use of negative staining is found to be more suitable for ultrastructural preservation in frozen sections compared to positive staining. The technique is demonstrated with various tissue preparations and suspensions of erythrocytes and bacterial cells, showing comparable or better morphological detail than conventional sections.