Received 3 November 1981; Revised and Accepted 17 November 1981 | Stephen M.Mount
The article by Stephen M. Mount presents a comprehensive catalogue of splice junction sequences from nuclear and viral genes encoding proteins. The study identifies consensus sequences for donor and acceptor splice sites, which are crucial for RNA splicing. The consensus sequence for donor sites is $\mathrm{C}_{\mathrm{A}}^{\mathrm{AG}} / \mathrm{GT}_{\mathrm{G}}^{\mathrm{AGT}}$, and for acceptor sites, it is $(\mathrm{T}_{\mathrm{C}}^{\mathrm{T}} \mathrm{N}_{\mathrm{T}}^{\mathrm{C}})^{\mathrm{AG}} / \mathrm{G}$. These sequences are derived from a large number of sequences and show high conservation across diverse species. The article discusses the implications of these consensus sequences for the splicing process and suggests that they may serve as signals for processing. Additionally, the study examines the frequency of repeat lengths at donor and acceptor sites, finding that the observed frequencies generally align with the expected frequencies, supporting the idea that splicing is precise. The results highlight the importance of these consensus sequences in ensuring accurate RNA splicing.The article by Stephen M. Mount presents a comprehensive catalogue of splice junction sequences from nuclear and viral genes encoding proteins. The study identifies consensus sequences for donor and acceptor splice sites, which are crucial for RNA splicing. The consensus sequence for donor sites is $\mathrm{C}_{\mathrm{A}}^{\mathrm{AG}} / \mathrm{GT}_{\mathrm{G}}^{\mathrm{AGT}}$, and for acceptor sites, it is $(\mathrm{T}_{\mathrm{C}}^{\mathrm{T}} \mathrm{N}_{\mathrm{T}}^{\mathrm{C}})^{\mathrm{AG}} / \mathrm{G}$. These sequences are derived from a large number of sequences and show high conservation across diverse species. The article discusses the implications of these consensus sequences for the splicing process and suggests that they may serve as signals for processing. Additionally, the study examines the frequency of repeat lengths at donor and acceptor sites, finding that the observed frequencies generally align with the expected frequencies, supporting the idea that splicing is precise. The results highlight the importance of these consensus sequences in ensuring accurate RNA splicing.