February 20, 2015 | Nicholas J. Loman1, Joshua Quick1 & Jared T. Simpson2
The authors present a method for de novo assembly of bacterial genomes using only Oxford Nanopore MinION sequencing data. They demonstrate that this approach can reconstruct the entire chromosome of *Escherichia coli* K-12 MG1655 into a single contig of 4.6 Mb, achieving 98.4% nucleotide identity with the reference genome. The method involves detecting overlaps between nanopore reads, error correction using partial order graphs, and assembly with the Celera assembler. The study highlights the potential of long-read nanopore sequencing for high-contiguity genome assemblies without the need for complementary sequencing technologies. The authors also discuss the limitations and future improvements, such as further refining the error profile of the assembly.The authors present a method for de novo assembly of bacterial genomes using only Oxford Nanopore MinION sequencing data. They demonstrate that this approach can reconstruct the entire chromosome of *Escherichia coli* K-12 MG1655 into a single contig of 4.6 Mb, achieving 98.4% nucleotide identity with the reference genome. The method involves detecting overlaps between nanopore reads, error correction using partial order graphs, and assembly with the Celera assembler. The study highlights the potential of long-read nanopore sequencing for high-contiguity genome assemblies without the need for complementary sequencing technologies. The authors also discuss the limitations and future improvements, such as further refining the error profile of the assembly.