This study reports the discovery of a lymphatic vessel network in the dura mater of the mouse brain, which drains cerebrospinal fluid (CSF) and brain interstitial fluid (ISF) into deep cervical lymph nodes (dCLNs). The dural lymphatic vessels are lined by fully differentiated lymphatic endothelium and are unique in their morphology and scarcity of valves. The authors found that these vessels absorb CSF from the subarachnoid space and brain ISF, transporting it into dCLNs via foramina at the base of the skull. In a transgenic mouse model with impaired dural lymphatic vessel development, macromolecule clearance from the brain was reduced, and transport into dCLNs was abrogated, despite normal brain ISF pressure and water content. These findings suggest that the dural lymphatic network plays a crucial role in the clearance of macromolecules from the brain and may be important for the management of brain waste products. The study also highlights the need to reevaluate the role of the lymphatic system in central nervous system physiology and disease.This study reports the discovery of a lymphatic vessel network in the dura mater of the mouse brain, which drains cerebrospinal fluid (CSF) and brain interstitial fluid (ISF) into deep cervical lymph nodes (dCLNs). The dural lymphatic vessels are lined by fully differentiated lymphatic endothelium and are unique in their morphology and scarcity of valves. The authors found that these vessels absorb CSF from the subarachnoid space and brain ISF, transporting it into dCLNs via foramina at the base of the skull. In a transgenic mouse model with impaired dural lymphatic vessel development, macromolecule clearance from the brain was reduced, and transport into dCLNs was abrogated, despite normal brain ISF pressure and water content. These findings suggest that the dural lymphatic network plays a crucial role in the clearance of macromolecules from the brain and may be important for the management of brain waste products. The study also highlights the need to reevaluate the role of the lymphatic system in central nervous system physiology and disease.