The article discusses the determination of the ester sulfate content of polysaccharide sulfates, focusing on methods for estimating sulfate content. Traditional methods such as gravimetric, colorimetric, titration, and spectrophotometric approaches have limitations, including tediousness, insensitivity, or requiring high manipulative skill. The authors describe a turbidimetric method based on those by Gassner & Friedel (1956) and Berglund & Sörbo (1959), which measures light absorption at 360 nm using barium sulfate. They address issues like the formation of ultraviolet-absorbing products during hydrolysis, which can lead to falsely high sulfate estimates. The method involves hydrolyzing polysaccharide sulfates with acid at controlled conditions to minimize the formation of these products. The results obtained using this method are consistent with those from conventional gravimetric procedures. The study highlights the speed and simplicity of the new method, making it suitable for analyzing various polysaccharide sulfates, including those from plant and animal sources.The article discusses the determination of the ester sulfate content of polysaccharide sulfates, focusing on methods for estimating sulfate content. Traditional methods such as gravimetric, colorimetric, titration, and spectrophotometric approaches have limitations, including tediousness, insensitivity, or requiring high manipulative skill. The authors describe a turbidimetric method based on those by Gassner & Friedel (1956) and Berglund & Sörbo (1959), which measures light absorption at 360 nm using barium sulfate. They address issues like the formation of ultraviolet-absorbing products during hydrolysis, which can lead to falsely high sulfate estimates. The method involves hydrolyzing polysaccharide sulfates with acid at controlled conditions to minimize the formation of these products. The results obtained using this method are consistent with those from conventional gravimetric procedures. The study highlights the speed and simplicity of the new method, making it suitable for analyzing various polysaccharide sulfates, including those from plant and animal sources.