This paper presents a rapid and simple method for preparing RNA from *Saccharomyces cerevisiae* using phenol and SDS. The method is designed to be mini-prep-friendly, allowing for the processing of multiple samples in about 60 minutes and providing sufficient RNA for several northern blots. The procedure involves growing yeast cultures, harvesting and resuspending the cells, followed by a series of extraction steps using phenol and chloroform. The RNA is then precipitated with ethanol and stored at -70°C. The method yields RNA ranging from 60 to 300 μg per 10 ml culture, with an average yield of 135 μg. The quality of the RNA is confirmed by agarose gel electrophoresis and northern blot analysis, showing no degradation of ribosomal RNA bands and minimal mRNA degradation. This method is particularly useful for preparing RNA from various yeast strains and growth conditions, avoiding the need for expensive and time-consuming glass bead techniques.This paper presents a rapid and simple method for preparing RNA from *Saccharomyces cerevisiae* using phenol and SDS. The method is designed to be mini-prep-friendly, allowing for the processing of multiple samples in about 60 minutes and providing sufficient RNA for several northern blots. The procedure involves growing yeast cultures, harvesting and resuspending the cells, followed by a series of extraction steps using phenol and chloroform. The RNA is then precipitated with ethanol and stored at -70°C. The method yields RNA ranging from 60 to 300 μg per 10 ml culture, with an average yield of 135 μg. The quality of the RNA is confirmed by agarose gel electrophoresis and northern blot analysis, showing no degradation of ribosomal RNA bands and minimal mRNA degradation. This method is particularly useful for preparing RNA from various yeast strains and growth conditions, avoiding the need for expensive and time-consuming glass bead techniques.