The chapter reviews the current state of natural biomaterials in wound healing applications. It highlights the dynamic and complex nature of wound healing, which involves four stages: hemostasis, inflammation, proliferation, and regeneration. The text discusses the advantages of using natural biomaterials, such as their biomimicry of the extracellular matrix (ECM), accessibility, and involvement in native wound healing. However, limitations like low mechanical properties and expensive extraction methods are also noted. The combination of natural biomaterials with synthetic biomaterials and bioactive agents is proposed as a solution. The chapter then delves into the structure, extraction methods, and participation of selected natural biomaterials in wound healing. Key natural biomaterials discussed include polysaccharides, proteins, and bioactive agents. Polysaccharides such as silk, keratin, bacterial cellulose, hyaluronic acid (HA), N-acetylglucosamine, gelatin, β-glucan, dextran, chitin/chitosan, and collagen are highlighted for their potential in wound dressing applications. The text also covers the production methods, mechanical properties, and biological activities of these biomaterials. The chapter concludes by emphasizing the importance of considering various factors, including systemic and intrinsic/local factors, in wound healing. It provides a comprehensive overview of the wound healing process and the role of different biomaterials in promoting wound healing, particularly in chronic wounds. The discussion includes the use of biomaterials in different forms, such as hydrogels, films, nanofibers, and scaffolds, and their effectiveness in accelerating wound healing and preventing infections.The chapter reviews the current state of natural biomaterials in wound healing applications. It highlights the dynamic and complex nature of wound healing, which involves four stages: hemostasis, inflammation, proliferation, and regeneration. The text discusses the advantages of using natural biomaterials, such as their biomimicry of the extracellular matrix (ECM), accessibility, and involvement in native wound healing. However, limitations like low mechanical properties and expensive extraction methods are also noted. The combination of natural biomaterials with synthetic biomaterials and bioactive agents is proposed as a solution. The chapter then delves into the structure, extraction methods, and participation of selected natural biomaterials in wound healing. Key natural biomaterials discussed include polysaccharides, proteins, and bioactive agents. Polysaccharides such as silk, keratin, bacterial cellulose, hyaluronic acid (HA), N-acetylglucosamine, gelatin, β-glucan, dextran, chitin/chitosan, and collagen are highlighted for their potential in wound dressing applications. The text also covers the production methods, mechanical properties, and biological activities of these biomaterials. The chapter concludes by emphasizing the importance of considering various factors, including systemic and intrinsic/local factors, in wound healing. It provides a comprehensive overview of the wound healing process and the role of different biomaterials in promoting wound healing, particularly in chronic wounds. The discussion includes the use of biomaterials in different forms, such as hydrogels, films, nanofibers, and scaffolds, and their effectiveness in accelerating wound healing and preventing infections.