This comprehensive review explores the mechanisms of biofilm formation on various biomedical implants and devices, and discusses strategies to mitigate biofilm-associated infections. The study covers a wide range of implants and devices, including dental, orthopedic, cochlear, tracheal, breast, central venous, urinary, intraocular, and heart valves implants, as well as hemodialyzers and pacemakers. It highlights the importance of understanding biofilm formation, which is often caused by both Gram-positive and Gram-negative pathogens, and its consequences such as implant failure and chronic infections. The review critically examines various approaches to combat implant-associated infections, with a focus on non-antibiotic alternatives. These include the use of plant-derived compounds, nanoparticles, and antimicrobial peptides, which have shown promise in preventing bacterial colonization and biofilm formation. The article also discusses the challenges posed by antimicrobial resistance and the need for effective therapeutic strategies, emphasizing the potential of non-antibiotic compounds as effective implant coatings.This comprehensive review explores the mechanisms of biofilm formation on various biomedical implants and devices, and discusses strategies to mitigate biofilm-associated infections. The study covers a wide range of implants and devices, including dental, orthopedic, cochlear, tracheal, breast, central venous, urinary, intraocular, and heart valves implants, as well as hemodialyzers and pacemakers. It highlights the importance of understanding biofilm formation, which is often caused by both Gram-positive and Gram-negative pathogens, and its consequences such as implant failure and chronic infections. The review critically examines various approaches to combat implant-associated infections, with a focus on non-antibiotic alternatives. These include the use of plant-derived compounds, nanoparticles, and antimicrobial peptides, which have shown promise in preventing bacterial colonization and biofilm formation. The article also discusses the challenges posed by antimicrobial resistance and the need for effective therapeutic strategies, emphasizing the potential of non-antibiotic compounds as effective implant coatings.