This chapter describes a modified method for rapidly isolating total RNA from small amounts of plant leaf material, addressing the limitations of conventional methods that are time-consuming and prone to RNase contamination. The procedure involves grinding fresh leaf discs in liquid nitrogen, followed by extraction with hot buffer, chloroform, and isopropanol. The RNA is then precipitated and purified using lithium chloride and ethanol. This method allows for the simultaneous handling of multiple samples and yields 25 to 50 μg of total RNA from 100 mg of leaf tissue, suitable for Northern blot analysis.This chapter describes a modified method for rapidly isolating total RNA from small amounts of plant leaf material, addressing the limitations of conventional methods that are time-consuming and prone to RNase contamination. The procedure involves grinding fresh leaf discs in liquid nitrogen, followed by extraction with hot buffer, chloroform, and isopropanol. The RNA is then precipitated and purified using lithium chloride and ethanol. This method allows for the simultaneous handling of multiple samples and yields 25 to 50 μg of total RNA from 100 mg of leaf tissue, suitable for Northern blot analysis.