A rapid method for isolating plant RNA is described. The method allows the isolation of total RNA from as little as 100 mg of leaf material. The procedure uses eppendorf tubes to handle multiple samples simultaneously. Fresh leaf discs are collected, frozen in liquid nitrogen, and ground with a precooled steel bar. Extraction buffer is added, and the mixture is homogenized and treated with chloroform-isoamylalcohol. After centrifugation, the aqueous phase is mixed with LiCl, and RNA precipitates overnight. The RNA is then precipitated again with ethanol, washed, and dried. The method yields 25-50 µg of RNA from 100 mg of leaf tissue. The procedure is suitable for Northern blot analysis of transgenic plants. The method is efficient and reduces the risk of RNase contamination compared to traditional methods. The procedure was adapted from a previously described method. The method is suitable for the analysis of RNA levels in large numbers of plants. The method is simple and efficient, allowing for the rapid isolation of RNA from small leaf samples. The method is suitable for use with various plant species, including tobacco, tomato, and potato. The method is useful for the analysis of RNA levels in transgenic plants. The method is efficient and reduces the risk of RNase contamination. The method is suitable for the analysis of RNA levels in large numbers of plants. The method is simple and efficient, allowing for the rapid isolation of RNA from small leaf samples. The method is suitable for use with various plant species, including tobacco, tomato, and potato. The method is useful for the analysis of RNA levels in transgenic plants.A rapid method for isolating plant RNA is described. The method allows the isolation of total RNA from as little as 100 mg of leaf material. The procedure uses eppendorf tubes to handle multiple samples simultaneously. Fresh leaf discs are collected, frozen in liquid nitrogen, and ground with a precooled steel bar. Extraction buffer is added, and the mixture is homogenized and treated with chloroform-isoamylalcohol. After centrifugation, the aqueous phase is mixed with LiCl, and RNA precipitates overnight. The RNA is then precipitated again with ethanol, washed, and dried. The method yields 25-50 µg of RNA from 100 mg of leaf tissue. The procedure is suitable for Northern blot analysis of transgenic plants. The method is efficient and reduces the risk of RNase contamination compared to traditional methods. The procedure was adapted from a previously described method. The method is suitable for the analysis of RNA levels in large numbers of plants. The method is simple and efficient, allowing for the rapid isolation of RNA from small leaf samples. The method is suitable for use with various plant species, including tobacco, tomato, and potato. The method is useful for the analysis of RNA levels in transgenic plants. The method is efficient and reduces the risk of RNase contamination. The method is suitable for the analysis of RNA levels in large numbers of plants. The method is simple and efficient, allowing for the rapid isolation of RNA from small leaf samples. The method is suitable for use with various plant species, including tobacco, tomato, and potato. The method is useful for the analysis of RNA levels in transgenic plants.