A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing has been developed, offering robust and inducible expression of four optogenetic tools: ChR2-tdTomato, ChR2-EYFP, Arch-ER2, and eNpHR3.0. These mice enable precise manipulation of targeted neuronal activity in both in vitro and in vivo settings. The ChR2-EYFP and Arch-ER2 lines demonstrate light sensitivity comparable to in utero or viral transduced neurons. The study shows that these mice can effectively activate and silence specific neuronal populations, such as parvalbumin-positive interneurons, in behaving animals. The robust and inducible nature of these mice represents a significant advancement over previous lines, while the Arch-ER2 and eNpHR3.0 lines are the first to demonstrate successful conditional optogenetic silencing. When combined with available Cre-driver lines, these mice enable widespread investigations of neural circuits with unprecedented reliability and accuracy. The study also demonstrates effective light activation and silencing of cortical pyramidal neurons, as well as light inhibition of hippocampal network activity. The mice show consistent and reliable responses to various light sources, including red and white LEDs, and are effective for both in vitro and in vivo experiments. The results highlight the potential of these mice for optogenetic studies of brain circuitry and neural function. The study provides a valuable resource for researchers seeking to manipulate and study specific neuronal populations in the brain.A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing has been developed, offering robust and inducible expression of four optogenetic tools: ChR2-tdTomato, ChR2-EYFP, Arch-ER2, and eNpHR3.0. These mice enable precise manipulation of targeted neuronal activity in both in vitro and in vivo settings. The ChR2-EYFP and Arch-ER2 lines demonstrate light sensitivity comparable to in utero or viral transduced neurons. The study shows that these mice can effectively activate and silence specific neuronal populations, such as parvalbumin-positive interneurons, in behaving animals. The robust and inducible nature of these mice represents a significant advancement over previous lines, while the Arch-ER2 and eNpHR3.0 lines are the first to demonstrate successful conditional optogenetic silencing. When combined with available Cre-driver lines, these mice enable widespread investigations of neural circuits with unprecedented reliability and accuracy. The study also demonstrates effective light activation and silencing of cortical pyramidal neurons, as well as light inhibition of hippocampal network activity. The mice show consistent and reliable responses to various light sources, including red and white LEDs, and are effective for both in vitro and in vivo experiments. The results highlight the potential of these mice for optogenetic studies of brain circuitry and neural function. The study provides a valuable resource for researchers seeking to manipulate and study specific neuronal populations in the brain.