The paper presents an innovative method for producing structured cell-laden hydrogel fibers and tubules using acoustic cell patterning. This technique leverages the biocompatibility, tunability, and contact-free nature of acoustic manipulation to create biomimetic tissues with ordered cell arrangements. The system involves injecting pre-cross-linked hydrogel containing cells or microparticles into a glass capillary, where acoustic waves generated by a piezoelectric transducer create different resonance modes that arrange the cells or particles in specific patterns. The hydrogel is then cross-linked using UV light, forming structured fibers or tubules with the desired cell patterns. The study demonstrates the formation of 2/4/6-petal radiant cell assemblies at specific resonance frequencies and confirms good cell viability and proliferation over 72 hours. The method's biocompatibility and reliability make it suitable for various biomimetic applications, including tissue engineering and regenerative medicine.The paper presents an innovative method for producing structured cell-laden hydrogel fibers and tubules using acoustic cell patterning. This technique leverages the biocompatibility, tunability, and contact-free nature of acoustic manipulation to create biomimetic tissues with ordered cell arrangements. The system involves injecting pre-cross-linked hydrogel containing cells or microparticles into a glass capillary, where acoustic waves generated by a piezoelectric transducer create different resonance modes that arrange the cells or particles in specific patterns. The hydrogel is then cross-linked using UV light, forming structured fibers or tubules with the desired cell patterns. The study demonstrates the formation of 2/4/6-petal radiant cell assemblies at specific resonance frequencies and confirms good cell viability and proliferation over 72 hours. The method's biocompatibility and reliability make it suitable for various biomimetic applications, including tissue engineering and regenerative medicine.