Scanning Laser Ophthalmoscopy (SLO) is a diagnostic imaging technique that uses confocal laser scanning microscopy to image the retina or cornea of the human eye. SLO provides high spatial sensitivity, aiding in the diagnosis of conditions such as glaucoma and macular degeneration. However, it faces challenges with reflections from eye astigmatism and corneal issues, and eye movements can complicate data collection. Adaptive Optics Scanning Laser Ophthalmoscopy (AOSLO) enhances SLO by using adaptive optics to correct optical aberrations, improving lateral resolution and image quality. AOSLO has been instrumental in measuring living retinal cells, determining the spatial distribution of cone cells, and visualizing abnormalities in photoreceptor structure. It has also been used to track retinal damage from dystrophies and monitor blood flow in the eye. AOSLO offers several advantages over traditional methods like retinal dissection and other imaging techniques such as fluorescein angiography and optical coherence tomography (OCT). It provides real-time tracking of retinal changes, easier control of confounding factors, and high translational resolution, making it a valuable tool for clinical applications and research.Scanning Laser Ophthalmoscopy (SLO) is a diagnostic imaging technique that uses confocal laser scanning microscopy to image the retina or cornea of the human eye. SLO provides high spatial sensitivity, aiding in the diagnosis of conditions such as glaucoma and macular degeneration. However, it faces challenges with reflections from eye astigmatism and corneal issues, and eye movements can complicate data collection. Adaptive Optics Scanning Laser Ophthalmoscopy (AOSLO) enhances SLO by using adaptive optics to correct optical aberrations, improving lateral resolution and image quality. AOSLO has been instrumental in measuring living retinal cells, determining the spatial distribution of cone cells, and visualizing abnormalities in photoreceptor structure. It has also been used to track retinal damage from dystrophies and monitor blood flow in the eye. AOSLO offers several advantages over traditional methods like retinal dissection and other imaging techniques such as fluorescein angiography and optical coherence tomography (OCT). It provides real-time tracking of retinal changes, easier control of confounding factors, and high translational resolution, making it a valuable tool for clinical applications and research.