The article discusses the application of polylysine-coated surfaces for electron microscopy. Polylysine, a polycationic molecule, strongly adsorbs to solid surfaces, creating an environment where cells can adhere and spread. This method is particularly useful for maintaining living cells during experimental procedures, such as fertilization, and for preparing samples for scanning and transmission electron microscopy. The cells remain attached even after fixation and processing, allowing for detailed observation of subcellular structures. The technique has been successfully applied to various cell types, including Dictyostelium amebas and sea urchin eggs, and has been used to study the inner surfaces of cells and the behavior of subcellular structures. The authors also describe specific experimental procedures, such as treating flagella with Triton X-100, which can be performed on cells adhered to polylysine-coated surfaces.The article discusses the application of polylysine-coated surfaces for electron microscopy. Polylysine, a polycationic molecule, strongly adsorbs to solid surfaces, creating an environment where cells can adhere and spread. This method is particularly useful for maintaining living cells during experimental procedures, such as fertilization, and for preparing samples for scanning and transmission electron microscopy. The cells remain attached even after fixation and processing, allowing for detailed observation of subcellular structures. The technique has been successfully applied to various cell types, including Dictyostelium amebas and sea urchin eggs, and has been used to study the inner surfaces of cells and the behavior of subcellular structures. The authors also describe specific experimental procedures, such as treating flagella with Triton X-100, which can be performed on cells adhered to polylysine-coated surfaces.