Loop-mediated isothermal amplification (LAMP) is a molecular diagnostic technology introduced by Notomi et al. in 2000, enabling rapid nucleic acid amplification at a constant temperature. Unlike conventional PCR, which requires temperature cycling, LAMP achieves continuous rapid amplification under isothermal conditions using strand-displacing DNA polymerase and a set of four specific primers targeting six regions of the target gene. This design allows for high sensitivity, enabling amplification from a very small number of copies. The results of LAMP can be detected through turbidimetry, fluorometry, and colorimetry, making it suitable for on-site testing. LAMP's isothermal nature eliminates the need for expensive thermal cyclers, making the technique more accessible, especially in resource-limited settings. Its high sensitivity and rapid amplification capabilities make it particularly useful for detecting low-abundance nucleic acids in clinical, agricultural, and environmental samples. However, LAMP has limitations, including the risk of non-specific amplification and the inability to amplify short gene sequences. To address these challenges, significant advancements have been made in LAMP technology, focusing on improving primer design and DNA polymerase modification to enhance specificity and efficiency. Additionally, the integration of LAMP with innovative molecular techniques and biosensors has expanded its capabilities. LAMP technology has been widely applied in various fields, including food safety, animal and plant pathogens, and human diseases. It has been used to detect food-borne pathogens, genetically modified organisms, and counterfeit foods. In animal and plant pathogens, LAMP has been effective in detecting invasive species and viruses. In human diseases, LAMP has been used for rapid detection of pathogenic bacteria, such as *Campylobacter jejuni* and *Mycobacterium tuberculosis*. LAMP technology has undergone significant developments and applications, particularly in primer design and polymerase engineering. The integration of LAMP with other techniques, such as RCA, RPA, CRISPR-Cas systems, biosensors, and microfluidic technology, has further expanded its utility. These advancements have made detection devices smaller and more portable, making them suitable for point-of-care testing (POCT) in areas with limited medical resources. The continuous improvement of LAMP technology holds promise for addressing emerging challenges in healthcare, agriculture, and environmental monitoring.Loop-mediated isothermal amplification (LAMP) is a molecular diagnostic technology introduced by Notomi et al. in 2000, enabling rapid nucleic acid amplification at a constant temperature. Unlike conventional PCR, which requires temperature cycling, LAMP achieves continuous rapid amplification under isothermal conditions using strand-displacing DNA polymerase and a set of four specific primers targeting six regions of the target gene. This design allows for high sensitivity, enabling amplification from a very small number of copies. The results of LAMP can be detected through turbidimetry, fluorometry, and colorimetry, making it suitable for on-site testing. LAMP's isothermal nature eliminates the need for expensive thermal cyclers, making the technique more accessible, especially in resource-limited settings. Its high sensitivity and rapid amplification capabilities make it particularly useful for detecting low-abundance nucleic acids in clinical, agricultural, and environmental samples. However, LAMP has limitations, including the risk of non-specific amplification and the inability to amplify short gene sequences. To address these challenges, significant advancements have been made in LAMP technology, focusing on improving primer design and DNA polymerase modification to enhance specificity and efficiency. Additionally, the integration of LAMP with innovative molecular techniques and biosensors has expanded its capabilities. LAMP technology has been widely applied in various fields, including food safety, animal and plant pathogens, and human diseases. It has been used to detect food-borne pathogens, genetically modified organisms, and counterfeit foods. In animal and plant pathogens, LAMP has been effective in detecting invasive species and viruses. In human diseases, LAMP has been used for rapid detection of pathogenic bacteria, such as *Campylobacter jejuni* and *Mycobacterium tuberculosis*. LAMP technology has undergone significant developments and applications, particularly in primer design and polymerase engineering. The integration of LAMP with other techniques, such as RCA, RPA, CRISPR-Cas systems, biosensors, and microfluidic technology, has further expanded its utility. These advancements have made detection devices smaller and more portable, making them suitable for point-of-care testing (POCT) in areas with limited medical resources. The continuous improvement of LAMP technology holds promise for addressing emerging challenges in healthcare, agriculture, and environmental monitoring.