Loop-mediated isothermal amplification (LAMP) is a nucleic acid detection method known for its isothermal properties, high efficiency, sensitivity, and specificity. It uses 4–6 primers targeting 6–8 regions of the desired sequence, allowing amplification at 60–65°C and producing up to 10⁹ copies within one hour. The product can be monitored via turbidimetry, fluorometry, and colorimetry. However, LAMP faces challenges such as non-specific amplification, primer design difficulties, unsuitability for short gene sequences, and limited multiplexing. Recent advancements in polymerase and primer design have improved LAMP's speed and convenience. Integration with technologies like rolling circle amplification (RCA), recombinase polymerase amplification (RPA), and CRISPR-Cas systems has enhanced its efficiency. Combining LAMP with biosensors enables real-time analysis, expanding its use in point-of-care testing (POCT). Microfluidic technology has facilitated automation and miniaturization, allowing simultaneous detection of multiple targets and reducing contamination. This review highlights advancements in LAMP, focusing on primer design, polymerase engineering, and integration with other technologies. Continuous improvements and integration with complementary technologies have significantly enhanced LAMP's diagnostic capabilities, making it a robust tool for rapid, sensitive, and specific nucleic acid detection with promising implications for healthcare, agriculture, and environmental monitoring.Loop-mediated isothermal amplification (LAMP) is a nucleic acid detection method known for its isothermal properties, high efficiency, sensitivity, and specificity. It uses 4–6 primers targeting 6–8 regions of the desired sequence, allowing amplification at 60–65°C and producing up to 10⁹ copies within one hour. The product can be monitored via turbidimetry, fluorometry, and colorimetry. However, LAMP faces challenges such as non-specific amplification, primer design difficulties, unsuitability for short gene sequences, and limited multiplexing. Recent advancements in polymerase and primer design have improved LAMP's speed and convenience. Integration with technologies like rolling circle amplification (RCA), recombinase polymerase amplification (RPA), and CRISPR-Cas systems has enhanced its efficiency. Combining LAMP with biosensors enables real-time analysis, expanding its use in point-of-care testing (POCT). Microfluidic technology has facilitated automation and miniaturization, allowing simultaneous detection of multiple targets and reducing contamination. This review highlights advancements in LAMP, focusing on primer design, polymerase engineering, and integration with other technologies. Continuous improvements and integration with complementary technologies have significantly enhanced LAMP's diagnostic capabilities, making it a robust tool for rapid, sensitive, and specific nucleic acid detection with promising implications for healthcare, agriculture, and environmental monitoring.