This review provides a comprehensive overview of the application of magnetic nanomaterials in enzyme immobilization, highlighting their advantages and potential industrial uses. Magnetic nanomaterials, such as magnetite (Fe₂O₄), are chosen for their versatility, large surface area, and superparamagnetic properties, which facilitate easy separation and reuse in industrial processes. The review covers various methods of enzyme immobilization, including adsorption, covalent bonding, crosslinking, and encapsulation, each with its own advantages and challenges. Superparamagnetism, a key property of magnetic nanoparticles, allows for efficient separation and reusability of enzymes under external magnetic fields. The synthesis methods for magnetic nanoparticles, such as coprecipitation, sol-gel, hydrothermal synthesis, and thermal decomposition, are discussed, along with their impact on particle size, morphology, and magnetic properties. Structural characterizations techniques, including X-ray diffraction, Mössbauer spectroscopy, and energy dispersive X-ray analysis, are essential for understanding the properties of magnetic nanoparticles. The review also highlights the successful immobilization of various enzymes on magnetic nanoparticles, demonstrating improved stability, catalytic activity, and reusability. Finally, the review explores the potential of magnetic nanomaterials in bioreactors and their role in sustainable industrial applications, emphasizing the importance of optimizing enzyme immobilization protocols for efficient and sustainable biocatalysis.This review provides a comprehensive overview of the application of magnetic nanomaterials in enzyme immobilization, highlighting their advantages and potential industrial uses. Magnetic nanomaterials, such as magnetite (Fe₂O₄), are chosen for their versatility, large surface area, and superparamagnetic properties, which facilitate easy separation and reuse in industrial processes. The review covers various methods of enzyme immobilization, including adsorption, covalent bonding, crosslinking, and encapsulation, each with its own advantages and challenges. Superparamagnetism, a key property of magnetic nanoparticles, allows for efficient separation and reusability of enzymes under external magnetic fields. The synthesis methods for magnetic nanoparticles, such as coprecipitation, sol-gel, hydrothermal synthesis, and thermal decomposition, are discussed, along with their impact on particle size, morphology, and magnetic properties. Structural characterizations techniques, including X-ray diffraction, Mössbauer spectroscopy, and energy dispersive X-ray analysis, are essential for understanding the properties of magnetic nanoparticles. The review also highlights the successful immobilization of various enzymes on magnetic nanoparticles, demonstrating improved stability, catalytic activity, and reusability. Finally, the review explores the potential of magnetic nanomaterials in bioreactors and their role in sustainable industrial applications, emphasizing the importance of optimizing enzyme immobilization protocols for efficient and sustainable biocatalysis.