The article reviews recent advances in 3D bioprinting techniques for regenerative medicine applications, focusing on the fabrication of biocompatible and biofunctional structures using biomaterials, cells, and biomolecules. 3D bioprinting, an emerging method, uses biomaterial-based mixtures with cells and other biological constituents to create hydrogel-based objects with specific architectures and geometrical properties, promoting cell growth and differentiation. Various water-soluble biomaterials, including natural and synthetic biopolymers, and inorganic materials, are employed to prepare bioinks. The review highlights the importance of optimal biological response, mechanical properties, reproducibility, printing fidelity, and upscaling capability for successful applications. It discusses co-culture bioprinting systems that mimic the complexity of tissues and organs, and the basic physical principles governing 3D bioprinting. The article also examines the ideal bioink properties and the current status of 3D bioprinting, addressing its limitations and potential for producing functional artificial organs. Specific applications in bone, cartilage, cardiovascular, neural, skin, and other organ regeneration are detailed, along with the use of biomaterials such as hydroxyapatite, graphene oxide, and silk fibroin. The review concludes by discussing the challenges and future directions in 3D bioprinting for regenerative medicine.The article reviews recent advances in 3D bioprinting techniques for regenerative medicine applications, focusing on the fabrication of biocompatible and biofunctional structures using biomaterials, cells, and biomolecules. 3D bioprinting, an emerging method, uses biomaterial-based mixtures with cells and other biological constituents to create hydrogel-based objects with specific architectures and geometrical properties, promoting cell growth and differentiation. Various water-soluble biomaterials, including natural and synthetic biopolymers, and inorganic materials, are employed to prepare bioinks. The review highlights the importance of optimal biological response, mechanical properties, reproducibility, printing fidelity, and upscaling capability for successful applications. It discusses co-culture bioprinting systems that mimic the complexity of tissues and organs, and the basic physical principles governing 3D bioprinting. The article also examines the ideal bioink properties and the current status of 3D bioprinting, addressing its limitations and potential for producing functional artificial organs. Specific applications in bone, cartilage, cardiovascular, neural, skin, and other organ regeneration are detailed, along with the use of biomaterials such as hydroxyapatite, graphene oxide, and silk fibroin. The review concludes by discussing the challenges and future directions in 3D bioprinting for regenerative medicine.