This review discusses the recent advances in the application of transition-metal composite nanozymes in biomedicine. Natural enzymes have limitations in harsh environments, but nanozymes, which mimic enzymatic activity, can function in more extreme conditions. Composite nanozymes, made from noble metals like Au, Ag, and Pt and other transition metals, offer enhanced catalytic activity and stability. These nanozymes are being used in biomedical applications such as glucose detection, cancer diagnosis and treatment, and antibacterial therapy. Ag-based composite nanozymes have been used for disease diagnosis and antimicrobial applications. For example, Ag nanoparticles have been used to develop biosensors for detecting prostate-specific antigens and for antimicrobial therapy by generating reactive oxygen species. Ag-based nanozymes also show promise in glucose detection, where they can detect glucose in human serum with high sensitivity. Au-based composite nanozymes have been used for antimicrobial applications and glucose detection. These nanozymes can generate reactive oxygen species and photothermal effects, which can kill bacteria and cancer cells. Au-based nanozymes have also been used in cancer cell detection, where they can detect cancer cells through peroxidase-like activity. Pt-based composite nanozymes have been used for antimicrobial applications and tumor cell inactivation. These nanozymes can generate reactive oxygen species and photothermal effects, which can kill bacteria and cancer cells. Pt-based nanozymes have also been used in mercury ion detection and tumor cell therapy. Other transition-metal composite nanozymes have been used for various biomedical applications, including tumor cell inhibition and cancer cell detection. These nanozymes can generate reactive oxygen species and photothermal effects, which can kill cancer cells and inhibit tumor growth. The review highlights the potential of transition-metal composite nanozymes in biomedicine, emphasizing their ability to mimic enzymatic activity, their stability, and their potential for various biomedical applications. However, challenges remain in terms of cost, biocompatibility, and the need for further research to optimize their performance. The development of these nanozymes is an important area of research in biomedicine.This review discusses the recent advances in the application of transition-metal composite nanozymes in biomedicine. Natural enzymes have limitations in harsh environments, but nanozymes, which mimic enzymatic activity, can function in more extreme conditions. Composite nanozymes, made from noble metals like Au, Ag, and Pt and other transition metals, offer enhanced catalytic activity and stability. These nanozymes are being used in biomedical applications such as glucose detection, cancer diagnosis and treatment, and antibacterial therapy. Ag-based composite nanozymes have been used for disease diagnosis and antimicrobial applications. For example, Ag nanoparticles have been used to develop biosensors for detecting prostate-specific antigens and for antimicrobial therapy by generating reactive oxygen species. Ag-based nanozymes also show promise in glucose detection, where they can detect glucose in human serum with high sensitivity. Au-based composite nanozymes have been used for antimicrobial applications and glucose detection. These nanozymes can generate reactive oxygen species and photothermal effects, which can kill bacteria and cancer cells. Au-based nanozymes have also been used in cancer cell detection, where they can detect cancer cells through peroxidase-like activity. Pt-based composite nanozymes have been used for antimicrobial applications and tumor cell inactivation. These nanozymes can generate reactive oxygen species and photothermal effects, which can kill bacteria and cancer cells. Pt-based nanozymes have also been used in mercury ion detection and tumor cell therapy. Other transition-metal composite nanozymes have been used for various biomedical applications, including tumor cell inhibition and cancer cell detection. These nanozymes can generate reactive oxygen species and photothermal effects, which can kill cancer cells and inhibit tumor growth. The review highlights the potential of transition-metal composite nanozymes in biomedicine, emphasizing their ability to mimic enzymatic activity, their stability, and their potential for various biomedical applications. However, challenges remain in terms of cost, biocompatibility, and the need for further research to optimize their performance. The development of these nanozymes is an important area of research in biomedicine.