Platelet-rich plasma (PRP) has emerged as a promising adjunct in bone healing, though its clinical efficacy remains debated. Despite pre-clinical studies showing potential benefits in promoting bone regeneration through growth factors like PDGF, TGF-β, and IGF-1, clinical trials have not conclusively demonstrated its effectiveness. PRP's efficacy is influenced by factors such as preparation techniques, platelet and leukocyte concentrations, and activation methods. Variability in PRP formulations and protocols complicates its clinical application, as no standardized method has been established. While PRP may enhance bone healing, its use in non-union treatment is not universally supported due to inconsistent study results. The biological activity of PRP involves modulating inflammation, promoting angiogenesis, and facilitating tissue repair through growth factors and cytokines. However, the optimal concentration and delivery method of PRP remain unclear, with studies showing both positive and negative outcomes depending on the PRP type and application. Clinical studies have shown mixed results, with some reporting improved bone healing and others indicating no significant benefit. The integration of PRP into clinical practice requires further research to establish standardized protocols and confirm its therapeutic potential. Overall, PRP holds promise for bone healing but requires more rigorous studies to determine its true efficacy and optimal use in clinical settings.Platelet-rich plasma (PRP) has emerged as a promising adjunct in bone healing, though its clinical efficacy remains debated. Despite pre-clinical studies showing potential benefits in promoting bone regeneration through growth factors like PDGF, TGF-β, and IGF-1, clinical trials have not conclusively demonstrated its effectiveness. PRP's efficacy is influenced by factors such as preparation techniques, platelet and leukocyte concentrations, and activation methods. Variability in PRP formulations and protocols complicates its clinical application, as no standardized method has been established. While PRP may enhance bone healing, its use in non-union treatment is not universally supported due to inconsistent study results. The biological activity of PRP involves modulating inflammation, promoting angiogenesis, and facilitating tissue repair through growth factors and cytokines. However, the optimal concentration and delivery method of PRP remain unclear, with studies showing both positive and negative outcomes depending on the PRP type and application. Clinical studies have shown mixed results, with some reporting improved bone healing and others indicating no significant benefit. The integration of PRP into clinical practice requires further research to establish standardized protocols and confirm its therapeutic potential. Overall, PRP holds promise for bone healing but requires more rigorous studies to determine its true efficacy and optimal use in clinical settings.