The article reviews the advancements in organoid engineering for tissue regeneration and biofunctional reconstruction. It highlights the clinical challenges posed by tissue damage and functional abnormalities, emphasizing the limitations of traditional stem cell therapies. The review discusses the potential of engineered organoids, which can overcome these challenges by leveraging bioengineering principles to enhance reproducibility, control, and efficiency in organoid culture. The authors describe various strategies for cultivating engineered organoids, including the use of microwell arrays, bioreactor systems, microfluidics, bioprinting, and hydrogel matrices. They also explore the biological characteristics of organoids derived from different sources, such as adult stem cells (ASCs) and pluripotent stem cells (PSCs), and discuss the advantages and limitations of each. The article further delves into the engineering of organoids at different levels, including cellular, niche, and organismal levels, to achieve more precise control over their development and function. Finally, it addresses the limitations and challenges in organoid engineering, such as ethical concerns, tumorigenicity, and the need for further research to improve organoid maturation and clinical translation. The review concludes by outlining future research directions and the potential for personalized tissue repair and biofunctional reconstruction using advanced bioengineering tools.The article reviews the advancements in organoid engineering for tissue regeneration and biofunctional reconstruction. It highlights the clinical challenges posed by tissue damage and functional abnormalities, emphasizing the limitations of traditional stem cell therapies. The review discusses the potential of engineered organoids, which can overcome these challenges by leveraging bioengineering principles to enhance reproducibility, control, and efficiency in organoid culture. The authors describe various strategies for cultivating engineered organoids, including the use of microwell arrays, bioreactor systems, microfluidics, bioprinting, and hydrogel matrices. They also explore the biological characteristics of organoids derived from different sources, such as adult stem cells (ASCs) and pluripotent stem cells (PSCs), and discuss the advantages and limitations of each. The article further delves into the engineering of organoids at different levels, including cellular, niche, and organismal levels, to achieve more precise control over their development and function. Finally, it addresses the limitations and challenges in organoid engineering, such as ethical concerns, tumorigenicity, and the need for further research to improve organoid maturation and clinical translation. The review concludes by outlining future research directions and the potential for personalized tissue repair and biofunctional reconstruction using advanced bioengineering tools.