Aerosol jet printing is used to fabricate surface acoustic wave (SAW) microfluidic devices, offering a rapid, cleanroom-free method for creating customizable devices. This technique enables the fabrication of SAW microfluidic devices with varying materials, including silver nanowires, graphene, and PEDOT:PSS. The devices were characterized using scanning laser Doppler vibrometry to measure wave displacements and resonant frequencies, demonstrating their acoustic performance. The devices were tested for acoustic streaming and particle concentration, showing their potential for lab-on-a-chip applications. The aerosol jet printing process allows for high-resolution, direct-write fabrication without the need for masks or complex cleanroom equipment. The method significantly reduces fabrication time and steps compared to traditional cleanroom techniques, enabling the rapid development of SAW microfluidic devices. The devices were shown to operate at frequencies ranging from 5 to 20 MHz, with varying electrode widths and materials. The study highlights the versatility and efficiency of aerosol jet printing in creating SAW microfluidic devices for applications in biology, chemistry, engineering, and medicine. The results demonstrate that aerosol jet printing provides a single-step, mask-free, and direct-write fabrication method for SAW microfluidic devices, offering a viable solution for rapid development and customization.Aerosol jet printing is used to fabricate surface acoustic wave (SAW) microfluidic devices, offering a rapid, cleanroom-free method for creating customizable devices. This technique enables the fabrication of SAW microfluidic devices with varying materials, including silver nanowires, graphene, and PEDOT:PSS. The devices were characterized using scanning laser Doppler vibrometry to measure wave displacements and resonant frequencies, demonstrating their acoustic performance. The devices were tested for acoustic streaming and particle concentration, showing their potential for lab-on-a-chip applications. The aerosol jet printing process allows for high-resolution, direct-write fabrication without the need for masks or complex cleanroom equipment. The method significantly reduces fabrication time and steps compared to traditional cleanroom techniques, enabling the rapid development of SAW microfluidic devices. The devices were shown to operate at frequencies ranging from 5 to 20 MHz, with varying electrode widths and materials. The study highlights the versatility and efficiency of aerosol jet printing in creating SAW microfluidic devices for applications in biology, chemistry, engineering, and medicine. The results demonstrate that aerosol jet printing provides a single-step, mask-free, and direct-write fabrication method for SAW microfluidic devices, offering a viable solution for rapid development and customization.