Ag-doped CNT/HAP nanohybrids in a PLLA bone scaffold show significant antibacterial activity

Ag-doped CNT/HAP nanohybrids in a PLLA bone scaffold show significant antibacterial activity

27 February 2024 | Cijun Shuai1,2,3 · Xiaoxin Shi1 · Kai Wang1 · Yulong Gu1 · Feng Yang1 · Pei Feng1
This study investigates the synthesis and antibacterial properties of Ag-doped CNT/HAP nanohybrids embedded in a PLLA bone scaffold. The researchers used an ion doping technique to partially replace calcium ions (Ca$^{2+}$) in the hydroxyapatite (HAP) lattice with silver ions (Ag$^{+}$) under hydrothermal conditions. This process involved the in situ synthesis of Ag-HAP nanoparticles on carbon nanotubes (CNTs). The resulting CNT/Ag-HAP nanohybrids were then incorporated into a PLLA matrix via laser-based powder bed fusion (PBF-LB) to create PLLA/CNT/Ag-HAP scaffolds. These scaffolds exhibited sustained antibacterial activity, with an effectiveness of 92.65%, due to the broad-spectrum antibacterial properties of Ag$^{+}$ and the physical destruction of bacterial structures by the sharp edges of CNTs. Additionally, the scaffolds showed enhanced mechanical properties, with tensile and compressive strengths of 8.49 MPa and 19.72 MPa, respectively. They also demonstrated good bioactivity and cytocompatibility, promoting the adhesion and proliferation of human osteoblast-like cells (MG63 cells). The study highlights the potential of Ag-doped CNT/HAP nanohybrids in enhancing the performance of bone scaffolds for clinical applications.This study investigates the synthesis and antibacterial properties of Ag-doped CNT/HAP nanohybrids embedded in a PLLA bone scaffold. The researchers used an ion doping technique to partially replace calcium ions (Ca$^{2+}$) in the hydroxyapatite (HAP) lattice with silver ions (Ag$^{+}$) under hydrothermal conditions. This process involved the in situ synthesis of Ag-HAP nanoparticles on carbon nanotubes (CNTs). The resulting CNT/Ag-HAP nanohybrids were then incorporated into a PLLA matrix via laser-based powder bed fusion (PBF-LB) to create PLLA/CNT/Ag-HAP scaffolds. These scaffolds exhibited sustained antibacterial activity, with an effectiveness of 92.65%, due to the broad-spectrum antibacterial properties of Ag$^{+}$ and the physical destruction of bacterial structures by the sharp edges of CNTs. Additionally, the scaffolds showed enhanced mechanical properties, with tensile and compressive strengths of 8.49 MPa and 19.72 MPa, respectively. They also demonstrated good bioactivity and cytocompatibility, promoting the adhesion and proliferation of human osteoblast-like cells (MG63 cells). The study highlights the potential of Ag-doped CNT/HAP nanohybrids in enhancing the performance of bone scaffolds for clinical applications.
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