This review discusses the use of alginate-based biomaterials in regenerative medicine, focusing on their applications in wound healing, cartilage repair, bone regeneration, and drug delivery. Alginate, a naturally occurring anionic polysaccharide, is known for its biocompatibility, biodegradability, and ability to form hydrogels, microspheres, sponges, foams, and fibers. These properties make it suitable for various biomedical applications, including tissue engineering and drug delivery systems. The review highlights the importance of modifying alginate to enhance its structural and functional properties. Techniques such as ionic crosslinking, phase transition, cell-crosslinking, free radical polymerization, and "click" reactions are discussed, each offering unique advantages in terms of degradation rate, mechanical strength, and cell adhesion. For example, ionic crosslinking with divalent cations like Ca²⁺ is a common method for preparing alginate hydrogels, while thermoresponsive phase transition allows for temperature-controlled gelation. Alginate-based microspheres and porous scaffolds are also explored for their potential in tissue engineering. Microspheres can encapsulate cells, growth factors, and bioactive proteins, while porous scaffolds provide a three-dimensional structure that supports cell growth and tissue regeneration. The review details the fabrication methods and applications of these materials, emphasizing their role in wound healing, cartilage repair, and bone regeneration. Finally, the review discusses the use of alginate-based biomaterials in drug delivery systems, highlighting their ability to encapsulate low-molecular-weight drugs and large biomacromolecules. The potential of alginate-based carriers in delivering bioactive signaling molecules, functional DNAs, and siRNAs is also discussed, along with their applications in tissue engineering and regenerative medicine. In conclusion, alginate-based biomaterials offer promising solutions for various regenerative medicine applications due to their biocompatibility, biodegradability, and versatility. However, further research is needed to optimize their properties for specific therapeutic uses.This review discusses the use of alginate-based biomaterials in regenerative medicine, focusing on their applications in wound healing, cartilage repair, bone regeneration, and drug delivery. Alginate, a naturally occurring anionic polysaccharide, is known for its biocompatibility, biodegradability, and ability to form hydrogels, microspheres, sponges, foams, and fibers. These properties make it suitable for various biomedical applications, including tissue engineering and drug delivery systems. The review highlights the importance of modifying alginate to enhance its structural and functional properties. Techniques such as ionic crosslinking, phase transition, cell-crosslinking, free radical polymerization, and "click" reactions are discussed, each offering unique advantages in terms of degradation rate, mechanical strength, and cell adhesion. For example, ionic crosslinking with divalent cations like Ca²⁺ is a common method for preparing alginate hydrogels, while thermoresponsive phase transition allows for temperature-controlled gelation. Alginate-based microspheres and porous scaffolds are also explored for their potential in tissue engineering. Microspheres can encapsulate cells, growth factors, and bioactive proteins, while porous scaffolds provide a three-dimensional structure that supports cell growth and tissue regeneration. The review details the fabrication methods and applications of these materials, emphasizing their role in wound healing, cartilage repair, and bone regeneration. Finally, the review discusses the use of alginate-based biomaterials in drug delivery systems, highlighting their ability to encapsulate low-molecular-weight drugs and large biomacromolecules. The potential of alginate-based carriers in delivering bioactive signaling molecules, functional DNAs, and siRNAs is also discussed, along with their applications in tissue engineering and regenerative medicine. In conclusion, alginate-based biomaterials offer promising solutions for various regenerative medicine applications due to their biocompatibility, biodegradability, and versatility. However, further research is needed to optimize their properties for specific therapeutic uses.