The OsciDrop digital PCR (dPCR) system is an innovative, all-in-one, chip-free platform designed to enhance the efficiency and multiplexing capabilities of dPCR. It eliminates the need for microfabricated chips, offering fully automated operations and improved multiplexing. The system integrates advanced techniques such as oscillation-induced droplet generation, precise liquid handling, pipette-based droplet printing, in situ thermocycling, multicolor fluorescence imaging, and machine learning-driven analysis. Key features include: 1. **OsciDrop Technique**: A novel method for generating monodisperse droplets using oscillating pipette tips, ensuring uniform droplet volume and number. 2. **Automated Workflow**: Streamlines the dPCR process, including droplet printing, thermocycling, and fluorescence imaging, with intelligent data processing. 3. **High Multiplexing**: Supports quadruplex dPCR assays for detecting key EGFR mutations in lung cancer and high-multiplex EGFR genotyping using digital stepwise melting analysis (dSMA). 4. **Cost-Effective and Versatile**: Offers cost-effective consumables and high-performance droplet generation, making it suitable for diverse clinical applications. 5. **Reliability and Precision**: Demonstrated by accurate quantification of reference materials and HER2 copy number variation in breast cancer. The OsciDrop system addresses the limitations of existing dPCR platforms, such as high consumable costs, complex procedures, and restricted multiplexing capabilities, and paves the way for transformative advances in precision diagnostics.The OsciDrop digital PCR (dPCR) system is an innovative, all-in-one, chip-free platform designed to enhance the efficiency and multiplexing capabilities of dPCR. It eliminates the need for microfabricated chips, offering fully automated operations and improved multiplexing. The system integrates advanced techniques such as oscillation-induced droplet generation, precise liquid handling, pipette-based droplet printing, in situ thermocycling, multicolor fluorescence imaging, and machine learning-driven analysis. Key features include: 1. **OsciDrop Technique**: A novel method for generating monodisperse droplets using oscillating pipette tips, ensuring uniform droplet volume and number. 2. **Automated Workflow**: Streamlines the dPCR process, including droplet printing, thermocycling, and fluorescence imaging, with intelligent data processing. 3. **High Multiplexing**: Supports quadruplex dPCR assays for detecting key EGFR mutations in lung cancer and high-multiplex EGFR genotyping using digital stepwise melting analysis (dSMA). 4. **Cost-Effective and Versatile**: Offers cost-effective consumables and high-performance droplet generation, making it suitable for diverse clinical applications. 5. **Reliability and Precision**: Demonstrated by accurate quantification of reference materials and HER2 copy number variation in breast cancer. The OsciDrop system addresses the limitations of existing dPCR platforms, such as high consumable costs, complex procedures, and restricted multiplexing capabilities, and paves the way for transformative advances in precision diagnostics.