A spatiotemporally hierarchical guided bone regeneration (GBR) membrane is developed by integrating a densely porous N-halamine functionalized bacterial cellulose nanonetwork facing the gingiva and a loosely porous chitosan-hydroxyapatite composite micronetwork facing the alveolar bone. This membrane asymmetrically combines stiffness and flexibility, ingrowth barrier and guiding, as well as antibacterial and cell-activation properties. The dense layer provides mechanical support, blocks fibroblasts and bacteria, and prevents bacterial invasion through multiple mechanisms. The loose layer conforms to bone surfaces, promotes osteogenesis, and creates a favorable osteogenic microenvironment. The membrane offers full protection during bone regeneration, effectively blocking bacterial invasion and promoting osteogenesis. The membrane's hierarchical structure and multi-dimensional antibacterial function provide comprehensive protection against bacteria. The membrane is compared to commercial collagen membranes, which lack sufficient antibacterial properties and are prone to degradation. The new membrane demonstrates superior mechanical properties, cytocompatibility, and pro-osteogenic ability. In vitro and in vivo tests show that the membrane effectively blocks bacterial invasion and promotes bone regeneration. The membrane's unique design enables it to overcome the limitations of traditional GBR membranes, making it a promising material for clinical bone augmentation.A spatiotemporally hierarchical guided bone regeneration (GBR) membrane is developed by integrating a densely porous N-halamine functionalized bacterial cellulose nanonetwork facing the gingiva and a loosely porous chitosan-hydroxyapatite composite micronetwork facing the alveolar bone. This membrane asymmetrically combines stiffness and flexibility, ingrowth barrier and guiding, as well as antibacterial and cell-activation properties. The dense layer provides mechanical support, blocks fibroblasts and bacteria, and prevents bacterial invasion through multiple mechanisms. The loose layer conforms to bone surfaces, promotes osteogenesis, and creates a favorable osteogenic microenvironment. The membrane offers full protection during bone regeneration, effectively blocking bacterial invasion and promoting osteogenesis. The membrane's hierarchical structure and multi-dimensional antibacterial function provide comprehensive protection against bacteria. The membrane is compared to commercial collagen membranes, which lack sufficient antibacterial properties and are prone to degradation. The new membrane demonstrates superior mechanical properties, cytocompatibility, and pro-osteogenic ability. In vitro and in vivo tests show that the membrane effectively blocks bacterial invasion and promotes bone regeneration. The membrane's unique design enables it to overcome the limitations of traditional GBR membranes, making it a promising material for clinical bone augmentation.