The study presents an innovative spatiotemporally hierarchical guided bone regeneration (GBR) membrane that combines densely porous N-halamine functionalized bacterial cellulose nanonetwork facing the gingiva and loosely porous chitosan-hydroxyapatite composite micronetwork facing the alveolar bone. This all-in-one membrane integrates stiffness and flexibility, ingrowth barrier and guidance, and antibacterial and cell-activation properties, providing comprehensive protection during bone regeneration. The dense layer, with a Young's modulus similar to that of the gingiva, maintains space for tissue regeneration while blocking fibroblasts and preventing bacterial invasion through multiple mechanisms. The loose layer, ultra-soft and conformal, facilitates osteogenesis by promoting the growth of osteoblasts. In vitro and in vivo tests demonstrate the membrane's superior cytocompatibility, barrier function, and pro-osteogenic ability, as well as its effectiveness in protecting against bacterial invasion. The membrane's multi-layered structure and multi-dimensional antibacterial function create a sterile osteogenic microenvironment, making it a promising material for clinical bone augmentation.The study presents an innovative spatiotemporally hierarchical guided bone regeneration (GBR) membrane that combines densely porous N-halamine functionalized bacterial cellulose nanonetwork facing the gingiva and loosely porous chitosan-hydroxyapatite composite micronetwork facing the alveolar bone. This all-in-one membrane integrates stiffness and flexibility, ingrowth barrier and guidance, and antibacterial and cell-activation properties, providing comprehensive protection during bone regeneration. The dense layer, with a Young's modulus similar to that of the gingiva, maintains space for tissue regeneration while blocking fibroblasts and preventing bacterial invasion through multiple mechanisms. The loose layer, ultra-soft and conformal, facilitates osteogenesis by promoting the growth of osteoblasts. In vitro and in vivo tests demonstrate the membrane's superior cytocompatibility, barrier function, and pro-osteogenic ability, as well as its effectiveness in protecting against bacterial invasion. The membrane's multi-layered structure and multi-dimensional antibacterial function create a sterile osteogenic microenvironment, making it a promising material for clinical bone augmentation.