This study investigates hydrogen production from biomass feedstocks (glucose, starch, lignin, and cellulose) using a 100 mL h-type proton exchange membrane electrolysis cell. The research employs FeCl₃ as a Lewis acid to aid in the depolymerization/oxidation of biomass components. The study measures H₂ and CO₂ emissions and performs electrochemical analysis to understand the process. The highest H₂ volume of 12.1 mL was achieved at ambient temperature with glucose, which was up to twice higher than other feedstocks at 1.20 V. Glucose also showed the maximum power-to-H₂ yield ratio of 30.99 kWh/kg. The results demonstrate that hydrogen can be produced from biomass feedstocks at ambient temperature using FeCl₃, with higher yield rates and lower electricity consumption compared to water electrolysis. The study provides benchmarking data for the research community, contributing to the development of low-carbon-emission hydrogen production from biomass resources.This study investigates hydrogen production from biomass feedstocks (glucose, starch, lignin, and cellulose) using a 100 mL h-type proton exchange membrane electrolysis cell. The research employs FeCl₃ as a Lewis acid to aid in the depolymerization/oxidation of biomass components. The study measures H₂ and CO₂ emissions and performs electrochemical analysis to understand the process. The highest H₂ volume of 12.1 mL was achieved at ambient temperature with glucose, which was up to twice higher than other feedstocks at 1.20 V. Glucose also showed the maximum power-to-H₂ yield ratio of 30.99 kWh/kg. The results demonstrate that hydrogen can be produced from biomass feedstocks at ambient temperature using FeCl₃, with higher yield rates and lower electricity consumption compared to water electrolysis. The study provides benchmarking data for the research community, contributing to the development of low-carbon-emission hydrogen production from biomass resources.