The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped various functional elements in the human genome, including transcription, transcription factor association, chromatin structure, and histone modification. These data have enabled the assignment of biochemical functions to 80% of the genome, particularly outside protein-coding regions. The project has identified many candidate regulatory elements that are physically associated with expressed genes and show statistical correspondence to sequence variants linked to human diseases. Overall, the ENCODE project provides new insights into the organization and regulation of the human genome and serves as an expansive resource for biomedical research. The project has also developed methods for integrating diverse experimental data and has identified a wide range of functional elements, including RNA, histone modifications, and transcription factor binding sites. The integration of these elements has revealed a complex landscape of gene-element connectivity and the impact of selection on functional elements. The ENCODE data have been used to develop predictive models for transcription levels and exon inclusion, and to explore the co-association between transcription factors. The project's findings have significant implications for understanding the human genome and its regulation.The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped various functional elements in the human genome, including transcription, transcription factor association, chromatin structure, and histone modification. These data have enabled the assignment of biochemical functions to 80% of the genome, particularly outside protein-coding regions. The project has identified many candidate regulatory elements that are physically associated with expressed genes and show statistical correspondence to sequence variants linked to human diseases. Overall, the ENCODE project provides new insights into the organization and regulation of the human genome and serves as an expansive resource for biomedical research. The project has also developed methods for integrating diverse experimental data and has identified a wide range of functional elements, including RNA, histone modifications, and transcription factor binding sites. The integration of these elements has revealed a complex landscape of gene-element connectivity and the impact of selection on functional elements. The ENCODE data have been used to develop predictive models for transcription levels and exon inclusion, and to explore the co-association between transcription factors. The project's findings have significant implications for understanding the human genome and its regulation.