This study investigates the occurrence of mycotoxins in plant-based beverages, which have gained popularity as alternatives to dairy milk. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and optimized to detect 22 mycotoxins in 72 plant-based beverages from Latvia and Lithuania. The method was validated according to European Commission regulations, with good linearity, recovery, and selectivity. The results showed that 64% of the beverages tested positive for one to sixteen mycotoxins, with enniatins, beauvericin, deoxynivalenol (DON), T-2, and HT-2 toxins being the most frequently detected. The study also assessed the exposure risk to mycotoxins from consuming plant-based beverages, particularly in the Latvian population, where dairy milk consumption is replaced by these beverages. The calculated probable daily intake (PDI) values for most mycotoxins were below the tolerable daily intake (TDI) levels, indicating relatively low acute health risks. However, exposure to certain mycotoxins, such as alternaria monomethyl ether (AME) and fumonisins, was not previously reported in plant-based beverages. The study concludes that plant-based beverages are generally safe for human consumption, but further research is needed to understand long-term effects and potential risks associated with mycotoxin exposure.This study investigates the occurrence of mycotoxins in plant-based beverages, which have gained popularity as alternatives to dairy milk. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and optimized to detect 22 mycotoxins in 72 plant-based beverages from Latvia and Lithuania. The method was validated according to European Commission regulations, with good linearity, recovery, and selectivity. The results showed that 64% of the beverages tested positive for one to sixteen mycotoxins, with enniatins, beauvericin, deoxynivalenol (DON), T-2, and HT-2 toxins being the most frequently detected. The study also assessed the exposure risk to mycotoxins from consuming plant-based beverages, particularly in the Latvian population, where dairy milk consumption is replaced by these beverages. The calculated probable daily intake (PDI) values for most mycotoxins were below the tolerable daily intake (TDI) levels, indicating relatively low acute health risks. However, exposure to certain mycotoxins, such as alternaria monomethyl ether (AME) and fumonisins, was not previously reported in plant-based beverages. The study concludes that plant-based beverages are generally safe for human consumption, but further research is needed to understand long-term effects and potential risks associated with mycotoxin exposure.