This study investigated the occurrence of mycotoxins in 72 plant-based beverages from Latvia and Lithuania using liquid chromatography–mass spectrometry. A total of 64% of the beverages tested were positive for mycotoxins, with deoxynivalenol, beauvericin, and enniatins being the most frequently detected. The study developed and validated a method for detecting 22 mycotoxins in plant-based beverages, including the use of QuEChERS extraction and ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS). The method showed good recovery, linearity, and precision, with quantification limits ranging from 0.0078 to 5.0 μg kg⁻¹. The study also assessed the potential exposure of the Latvian population to mycotoxins if plant-based beverages replaced dairy milk. The calculated probable daily intake (PDI) values for mycotoxins were generally low, with most values below the recommended tolerable daily intake (TDI). However, exposure to certain mycotoxins, such as AOH and AME, may require further investigation due to potential chronic health concerns. The study found that oat drinks had the highest levels of deoxynivalenol, while nut drinks had the highest levels of aflatoxin B2. Thermal treatment of plant-based beverages reduced mycotoxin concentrations, with the highest reduction observed in coffee machine processing. Overall, the study concluded that plant-based beverages generally pose a low acute health risk for the Latvian population, but further research is needed to fully understand the long-term effects and potential risks associated with mycotoxin exposure. The study highlights the importance of monitoring mycotoxin levels in plant-based beverages to ensure their safety for consumption.This study investigated the occurrence of mycotoxins in 72 plant-based beverages from Latvia and Lithuania using liquid chromatography–mass spectrometry. A total of 64% of the beverages tested were positive for mycotoxins, with deoxynivalenol, beauvericin, and enniatins being the most frequently detected. The study developed and validated a method for detecting 22 mycotoxins in plant-based beverages, including the use of QuEChERS extraction and ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS). The method showed good recovery, linearity, and precision, with quantification limits ranging from 0.0078 to 5.0 μg kg⁻¹. The study also assessed the potential exposure of the Latvian population to mycotoxins if plant-based beverages replaced dairy milk. The calculated probable daily intake (PDI) values for mycotoxins were generally low, with most values below the recommended tolerable daily intake (TDI). However, exposure to certain mycotoxins, such as AOH and AME, may require further investigation due to potential chronic health concerns. The study found that oat drinks had the highest levels of deoxynivalenol, while nut drinks had the highest levels of aflatoxin B2. Thermal treatment of plant-based beverages reduced mycotoxin concentrations, with the highest reduction observed in coffee machine processing. Overall, the study concluded that plant-based beverages generally pose a low acute health risk for the Latvian population, but further research is needed to fully understand the long-term effects and potential risks associated with mycotoxin exposure. The study highlights the importance of monitoring mycotoxin levels in plant-based beverages to ensure their safety for consumption.