The article provides an overview of current detection methods for RNA methylation, a critical epitranscriptomic mechanism involved in various cellular processes. RNA methylation, particularly m6A, m7G, m1A, m5C, and m6Am, plays a significant role in regulating RNA stability, translation, splicing, and other processes. The detection methods are categorized into five types: antibody-based, digestion-based, ligation-based, hybridization-based, and direct RNA-based methods. Each method has its advantages and disadvantages, and the choice depends on factors such as the type of RNA, the abundance of methylation marks, and the required resolution. The article highlights the importance of these methods in understanding the dynamic nature of RNA methylation and its implications in health and disease. Additionally, it discusses the challenges and future directions in the field, emphasizing the need for more sensitive and cost-effective methods to advance research in RNA methylation.The article provides an overview of current detection methods for RNA methylation, a critical epitranscriptomic mechanism involved in various cellular processes. RNA methylation, particularly m6A, m7G, m1A, m5C, and m6Am, plays a significant role in regulating RNA stability, translation, splicing, and other processes. The detection methods are categorized into five types: antibody-based, digestion-based, ligation-based, hybridization-based, and direct RNA-based methods. Each method has its advantages and disadvantages, and the choice depends on factors such as the type of RNA, the abundance of methylation marks, and the required resolution. The article highlights the importance of these methods in understanding the dynamic nature of RNA methylation and its implications in health and disease. Additionally, it discusses the challenges and future directions in the field, emphasizing the need for more sensitive and cost-effective methods to advance research in RNA methylation.