This comprehensive literature review examines the clinical potential of stromal vascular fraction (SVF), a diverse cell mixture derived from adipose tissue, in regenerative medicine. SVF, which includes cells like pericytes, smooth muscle cells, and adipose-derived stem cells (ADSCs), has shown therapeutic efficacy in various medical disciplines due to its ability to differentiate and secrete growth factors. The review covers SVF's composition, action mechanisms, clinical applications, and future directions, based on a systematic literature search from January 2018 to June 2023. Sixteen articles met the inclusion criteria and were analyzed, highlighting SVF's positive outcomes in orthopedics, plastic and reconstructive surgery, cardiology, and wound healing. SVF has demonstrated improved graft survival, reduced pain, enhanced tissue regeneration, and immunomodulatory and anti-inflammatory properties. However, the review also notes limitations such as small sample sizes and lack of long-term data, emphasizing the need for further research to standardize protocols, optimize dosage and delivery methods, and conduct long-term clinical trials. The future of SVF in regenerative medicine holds promise, with potential applications in broader medical disciplines and personalized regenerative therapies.This comprehensive literature review examines the clinical potential of stromal vascular fraction (SVF), a diverse cell mixture derived from adipose tissue, in regenerative medicine. SVF, which includes cells like pericytes, smooth muscle cells, and adipose-derived stem cells (ADSCs), has shown therapeutic efficacy in various medical disciplines due to its ability to differentiate and secrete growth factors. The review covers SVF's composition, action mechanisms, clinical applications, and future directions, based on a systematic literature search from January 2018 to June 2023. Sixteen articles met the inclusion criteria and were analyzed, highlighting SVF's positive outcomes in orthopedics, plastic and reconstructive surgery, cardiology, and wound healing. SVF has demonstrated improved graft survival, reduced pain, enhanced tissue regeneration, and immunomodulatory and anti-inflammatory properties. However, the review also notes limitations such as small sample sizes and lack of long-term data, emphasizing the need for further research to standardize protocols, optimize dosage and delivery methods, and conduct long-term clinical trials. The future of SVF in regenerative medicine holds promise, with potential applications in broader medical disciplines and personalized regenerative therapies.