This review article explores the role of anoikis, an integrin-dependent form of cell death, in various physiological and pathological processes. Anoikis is defined as an intrinsic apoptosis triggered by integrin-mediated anchorage deficiency. The article highlights the importance of integrins in mediating ECM signaling and their role in regulating cell fate, tissue repair, inflammatory responses, cardiovascular diseases, tumor metastasis, and stem cell research. It discusses the molecular mechanisms underlying anoikis, including the involvement of integrin subunits and their heterodimers, and the cross-talk between physical and chemical signals. The article also reviews the impact of anoikis on cell culture models, stem cell engineering, and therapeutic interventions. Specifically, it examines how anoikis resistance affects cell survival, differentiation, and migration, and how targeting anoikis can be a promising strategy for overcoming therapeutic resistance and improving treatment outcomes. The review concludes by emphasizing the potential of integrin-based regulatory strategies and the need for further research to understand the complex mechanisms of anoikis in disease progression and treatment.This review article explores the role of anoikis, an integrin-dependent form of cell death, in various physiological and pathological processes. Anoikis is defined as an intrinsic apoptosis triggered by integrin-mediated anchorage deficiency. The article highlights the importance of integrins in mediating ECM signaling and their role in regulating cell fate, tissue repair, inflammatory responses, cardiovascular diseases, tumor metastasis, and stem cell research. It discusses the molecular mechanisms underlying anoikis, including the involvement of integrin subunits and their heterodimers, and the cross-talk between physical and chemical signals. The article also reviews the impact of anoikis on cell culture models, stem cell engineering, and therapeutic interventions. Specifically, it examines how anoikis resistance affects cell survival, differentiation, and migration, and how targeting anoikis can be a promising strategy for overcoming therapeutic resistance and improving treatment outcomes. The review concludes by emphasizing the potential of integrin-based regulatory strategies and the need for further research to understand the complex mechanisms of anoikis in disease progression and treatment.