Marine algae-derived bioactive compounds exhibit significant anti-biofilm activity by inhibiting cell attachment, reducing cell growth, interfering with quorum sensing pathways, and disrupting extracellular polymeric substances. This review discusses the mechanisms of biofilm formation, quorum sensing pathways, and the anti-biofilm properties of marine algae-derived natural products. Biofilms are complex microbial communities that form on surfaces and are resistant to antibiotics and antimicrobial agents. Quorum sensing, a cell-to-cell communication system, plays a crucial role in biofilm formation and is a key target for anti-biofilm strategies. Marine algae produce various bioactive compounds, including sulfated polysaccharides, carotenoids, lipids, and phlorotannins, which can inhibit biofilm formation by interfering with quorum sensing and disrupting microbial cell communication. Algae-derived compounds such as fucoidan, astaxanthin, and phlorotannins have been shown to inhibit biofilm formation in various bacteria, including Pseudomonas aeruginosa and Staphylococcus aureus. Additionally, marine algae extracts have been found to have anti-biofilm activity against foodborne pathogens such as Salmonella and Listeria. The use of marine algae-derived compounds as anti-biofilm agents is a promising approach for controlling biofilm-related infections and improving food safety.Marine algae-derived bioactive compounds exhibit significant anti-biofilm activity by inhibiting cell attachment, reducing cell growth, interfering with quorum sensing pathways, and disrupting extracellular polymeric substances. This review discusses the mechanisms of biofilm formation, quorum sensing pathways, and the anti-biofilm properties of marine algae-derived natural products. Biofilms are complex microbial communities that form on surfaces and are resistant to antibiotics and antimicrobial agents. Quorum sensing, a cell-to-cell communication system, plays a crucial role in biofilm formation and is a key target for anti-biofilm strategies. Marine algae produce various bioactive compounds, including sulfated polysaccharides, carotenoids, lipids, and phlorotannins, which can inhibit biofilm formation by interfering with quorum sensing and disrupting microbial cell communication. Algae-derived compounds such as fucoidan, astaxanthin, and phlorotannins have been shown to inhibit biofilm formation in various bacteria, including Pseudomonas aeruginosa and Staphylococcus aureus. Additionally, marine algae extracts have been found to have anti-biofilm activity against foodborne pathogens such as Salmonella and Listeria. The use of marine algae-derived compounds as anti-biofilm agents is a promising approach for controlling biofilm-related infections and improving food safety.